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Literature summary extracted from
- Purification and characterization of a DNA topoisomerase I from Bifidobacterium breve (1989), Agric. Biol. Chem., 53, 3357-3358.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.1 | actinomycin D | - |
Bifidobacterium breve |  |
| 5.6.2.1 | camptothecins | no inhibition | Bifidobacterium breve | |
| 5.6.2.1 | Ethidium bromide | - |
Bifidobacterium breve | |
| 5.6.2.1 | Na+ | above 150 mM | Bifidobacterium breve | |
| 5.6.2.1 | Neomycin sulfate | - |
Bifidobacterium breve | |
| 5.6.2.1 | single-stranded DNA | heat-denatured single-stranded DNA | Bifidobacterium breve | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.1 | Ca2+ | divalent cation required Ca2+ or Mg2+ | Bifidobacterium breve | |
| 5.6.2.1 | K+ | stimulates | Bifidobacterium breve | |
| 5.6.2.1 | Mg2+ | Mg2+ or Ca2+ required | Bifidobacterium breve | |
| 5.6.2.1 | Mg2+ | optimal concentration 5-15 mM | Bifidobacterium breve | |
| 5.6.2.1 | Mn2+ | stimulates | Bifidobacterium breve | |
| 5.6.2.1 | Na+ | stimulates, optimal concentration: 50-100 mM | Bifidobacterium breve | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.6.2.1 | Bifidobacterium breve | - |
- |
- |
| 5.6.2.1 | Bifidobacterium breve YIT4006 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.6.2.1 | - |
Bifidobacterium breve |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 5.6.2.1 | -20°C, stable for more than 1 year | Bifidobacterium breve |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.2.1 | supercoiled DNA | relaxation | Bifidobacterium breve | relaxed closed circular DNA | - |
? | |
| 5.6.2.1 | supercoiled DNA | specific for negatively supercoiled DNA, no activity with positively supercoiled DNA | Bifidobacterium breve | relaxed closed circular DNA | - |
? | |
| 5.6.2.1 | supercoiled DNA | relaxation | Bifidobacterium breve YIT4006 | relaxed closed circular DNA | - |
? | |
| 5.6.2.1 | supercoiled DNA | specific for negatively supercoiled DNA, no activity with positively supercoiled DNA | Bifidobacterium breve YIT4006 | relaxed closed circular DNA | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.6.2.1 | 6.5 | 7 | - |
Bifidobacterium breve |
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