Any feedback?
Literature summary extracted from
- DNA topoisomerases (1996), DNA Replication in eucaryotic cells, Cold Spring Harbor Laboratory Press, , 587-617.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.1 | additional information | enzyme is posttranslationally modified and presumably regulated by phosphorylation | eukaryota |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.6.2.2 | determination of crystal structure of fragments | eukaryota |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.1 | camptothecins | - |
eukaryota |  |
| 5.6.2.1 | camptothecins | - |
Vaccinia virus | |
| 5.6.2.1 | topotecan | - |
eukaryota | |
| 5.6.2.2 | 2-Nitroimidazoles | - |
eukaryota | |
| 5.6.2.2 | 9-Hydroxy-isoellipticine | - |
eukaryota | |
| 5.6.2.2 | Aclarubicin | - |
eukaryota | |
| 5.6.2.2 | Acridines | - |
eukaryota | |
| 5.6.2.2 | adriamycin | - |
eukaryota | |
| 5.6.2.2 | Epipodophyllotoxins | - |
eukaryota | |
| 5.6.2.2 | genistein | - |
eukaryota | |
| 5.6.2.2 | Merbarone | - |
eukaryota | |
| 5.6.2.2 | Mitoxantrone | - |
eukaryota | |
| 5.6.2.2 | quinolones | - |
eukaryota | |
| 5.6.2.2 | suramin | - |
eukaryota | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.6.2.1 | Mg2+ | no requirement for Mg2+ | eukaryota | |
| 5.6.2.1 | Mg2+ | no requirement for Mg2+ | Vaccinia virus | |
| 5.6.2.1 | Mg2+ | no requirement for Mg2+ | Rabbit fibroma virus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.2.1 | additional information | eukaryota | enzyme controls the topological state of DNA | ? | - |
? | |
| 5.6.2.1 | additional information | Vaccinia virus | enzyme controls the topological state of DNA | ? | - |
? | |
| 5.6.2.1 | additional information | Rabbit fibroma virus | enzyme controls the topological state of DNA | ? | - |
? | |
| 5.6.2.1 | additional information | eukaryota | plays a role in transcriptional elongation | ? | - |
? | |
| 5.6.2.1 | additional information | eukaryota | plays a crucial role in controlling the physiological functions of DNA | ? | - |
? | |
| 5.6.2.1 | additional information | eukaryota | plays a direct role in the regulation of transcriptional initiation by acting as a repressor of the basal-level transcription | ? | - |
? | |
| 5.6.2.2 | additional information | eukaryota | involvement in RNA polymerase II transcription | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.6.2.1 | eukaryota | - |
- |
- |
| 5.6.2.1 | Rabbit fibroma virus | - |
- |
- |
| 5.6.2.1 | Vaccinia virus | - |
- |
- |
| 5.6.2.2 | Bacteria | - |
- |
- |
| 5.6.2.2 | eukaryota | - |
- |
- |
| 5.6.2.2 | Homo sapiens | - |
- |
- |
| 5.6.2.2 | Mus musculus | - |
- |
- |
| 5.6.2.2 | Saccharomyces cerevisiae | - |
- |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 5.6.2.1 | ATP-independent breakage of single-stranded DNA, followed by passage and rejoining | acts by directing a nucleophilic attack from the hydroxyl group of an internal Tyr into a phosphodiester bond in the DNA backbone, resulting in nicking of one strand of the DNA double helix and formation of a covalent linkage between the 3'-phosphate and the Tyr hydroxyl group | eukaryota | |
| 5.6.2.2 | ATP-dependent breakage, passage and rejoining of double-stranded DNA | mechanistic studies | eukaryota |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.6.2.1 | additional information | enzyme controls the topological state of DNA | eukaryota | ? | - |
? | |
| 5.6.2.1 | additional information | enzyme controls the topological state of DNA | Vaccinia virus | ? | - |
? | |
| 5.6.2.1 | additional information | enzyme controls the topological state of DNA | Rabbit fibroma virus | ? | - |
? | |
| 5.6.2.1 | additional information | plays a role in transcriptional elongation | eukaryota | ? | - |
? | |
| 5.6.2.1 | additional information | plays a crucial role in controlling the physiological functions of DNA | eukaryota | ? | - |
? | |
| 5.6.2.1 | additional information | plays a direct role in the regulation of transcriptional initiation by acting as a repressor of the basal-level transcription | eukaryota | ? | - |
? | |
| 5.6.2.1 | supercoiled DNA | rather weak sequence specificity | eukaryota | relaxed closed circular DNA | - |
? | |
| 5.6.2.1 | supercoiled DNA | rather weak sequence specificity | Vaccinia virus | relaxed closed circular DNA | - |
? | |
| 5.6.2.1 | supercoiled DNA | rather weak sequence specificity | Rabbit fibroma virus | relaxed closed circular DNA | - |
? | |
| 5.6.2.1 | supercoiled DNA | when oligonucleotide DNA duplexes are used as substrates, the generated cleavage complex contains an active enzyme, which is able to ligate the cleaved DNA to a variety of DNA fragments containing a free 5'-OH end | Vaccinia virus | relaxed closed circular DNA | - |
? | |
| 5.6.2.1 | supercoiled DNA | single-strand of DNA double helix | eukaryota | relaxed closed circular DNA | - |
? | |
| 5.6.2.1 | supercoiled DNA | single-strand of DNA double helix | Vaccinia virus | relaxed closed circular DNA | - |
? | |
| 5.6.2.1 | supercoiled DNA | single-strand of DNA double helix | Rabbit fibroma virus | relaxed closed circular DNA | - |
? | |
| 5.6.2.1 | supercoiled DNA | relaxation | eukaryota | relaxed closed circular DNA | - |
? | |
| 5.6.2.1 | supercoiled DNA | relaxation | Vaccinia virus | relaxed closed circular DNA | - |
? | |
| 5.6.2.1 | supercoiled DNA | relaxation | Rabbit fibroma virus | relaxed closed circular DNA | - |
? | |
| 5.6.2.2 | additional information | involvement in RNA polymerase II transcription | eukaryota | ? | - |
? | |
| 5.6.2.2 | network of DNA rings + ATP + H2O | decatenation | Bacteria | monomeric DNA circles + ADP + phosphate | - |
? | |
| 5.6.2.2 | network of DNA rings + ATP + H2O | decatenation | eukaryota | monomeric DNA circles + ADP + phosphate | - |
? | |
| 5.6.2.2 | network of DNA rings + ATP + H2O | decatenation | Mus musculus | monomeric DNA circles + ADP + phosphate | - |
? | |
| 5.6.2.2 | network of DNA rings + ATP + H2O | decatenation | Homo sapiens | monomeric DNA circles + ADP + phosphate | - |
? | |
| 5.6.2.2 | network of DNA rings + ATP + H2O | decatenation | Saccharomyces cerevisiae | monomeric DNA circles + ADP + phosphate | - |
? | |
| 5.6.2.2 | supercoiled DNA + ATP + H2O | catenation | Bacteria | catenated DNA networks + ADP + phosphate | - |
? | |
| 5.6.2.2 | supercoiled DNA + ATP + H2O | catenation | eukaryota | catenated DNA networks + ADP + phosphate | - |
? | |
| 5.6.2.2 | supercoiled DNA + ATP + H2O | catenation | Mus musculus | catenated DNA networks + ADP + phosphate | - |
? | |
| 5.6.2.2 | supercoiled DNA + ATP + H2O | catenation | Homo sapiens | catenated DNA networks + ADP + phosphate | - |
? | |
| 5.6.2.2 | supercoiled DNA + ATP + H2O | catenation | Saccharomyces cerevisiae | catenated DNA networks + ADP + phosphate | - |
? | |
| 5.6.2.2 | supercoiled DNA + ATP + H2O | relaxation | Bacteria | relaxed DNA + ADP + phosphate | - |
? | |
| 5.6.2.2 | supercoiled DNA + ATP + H2O | relaxation | eukaryota | relaxed DNA + ADP + phosphate | - |
? | |
| 5.6.2.2 | supercoiled DNA + ATP + H2O | relaxation | Mus musculus | relaxed DNA + ADP + phosphate | - |
? | |
| 5.6.2.2 | supercoiled DNA + ATP + H2O | relaxation | Homo sapiens | relaxed DNA + ADP + phosphate | - |
? | |
| 5.6.2.2 | supercoiled DNA + ATP + H2O | relaxation | Saccharomyces cerevisiae | relaxed DNA + ADP + phosphate | - |
? | |
| 5.6.2.2 | supercoiled DNA + ATP + H2O | relaxation of negatively or positively supercoiled DNA | Bacteria | relaxed DNA + ADP + phosphate | - |
? | |
| 5.6.2.2 | supercoiled DNA + ATP + H2O | relaxation of negatively or positively supercoiled DNA | eukaryota | relaxed DNA + ADP + phosphate | - |
? | |
| 5.6.2.2 | supercoiled DNA + ATP + H2O | relaxation of negatively or positively supercoiled DNA | Saccharomyces cerevisiae | relaxed DNA + ADP + phosphate | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
