Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yamamoto, N.; Shishido, K.; Ogawa, H.; Sekiguchi, T.; Nosho, Y.
    Partial purification and characterization of type I DNA topoisomerase from Bacillus stearothermophilus (1985), J. Biochem., 97, 811-820.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.1 KCl can partially replace for NaCl in activation of enzyme forms MW 100000 and MW 110000 Geobacillus stearothermophilus
5.6.2.1 Na+ enzyme forms MW 100000 and MW 110000: inhibition above 300 mM NaCl. Enzyme form MW 150000: inhibition above 50 mM NaCl Geobacillus stearothermophilus
5.6.2.1 NH4Cl can partially replace for NaCl in activation of enzyme forms MW 100000 and MW 110000 Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.1 Ca2+ CaCl2 slightly increases activity of enzyme forms MW 100000 and MW 110000 in presence of 150 mM NaCl. Activates enzyme form MW 150000 to the same extent as MgCl2 Geobacillus stearothermophilus
5.6.2.1 Mg2+ enzyme forms MW 100000 and MW 110000: addition of both 5 mM Mg2+ and 5 mM NaCl markedly increases activity. Little activity in presence of 2.5-7.5 mM MgCl2 alone. Enzyme form MW 150000: only MgCl2 is required for full activity, maximal activation at 5 mM Geobacillus stearothermophilus
5.6.2.1 Mn2+ slightly increases activity of enzyme forms MW 100000 and MW 110000 in presence of 150 mM NaCl Geobacillus stearothermophilus
5.6.2.1 Na+ enzyme forms MW 100000 and MW 110000: addition of both 5 mM Mg2+ and 5 mM NaCl markedly increases activity, optimal NaCl concentration is 150-200 mM, inhibition above 300 mM Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.2.1 80000
-
 4 forms of DNA topoisomerase I with MW 80000, MW 100000, MW 110000, and MW 150000, PAGE, gel filtration Geobacillus stearothermophilus
5.6.2.1 100000
-
 4 forms of DNA topoisomerase I with MW 80000, MW 100000, MW 110000, and MW 150000, PAGE, gel filtration Geobacillus stearothermophilus
5.6.2.1 110000
-
 4 forms of DNA topoisomerase I with MW 80000, MW 100000, MW 110000, and MW 150000, PAGE, gel filtration Geobacillus stearothermophilus
5.6.2.1 150000
-
 4 forms of DNA topoisomerase I with MW 80000, MW 100000, MW 110000, and MW 150000, PAGE, gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.1 Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.1 partial Geobacillus stearothermophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.1 supercoiled DNA relaxation Geobacillus stearothermophilus relaxed closed circular DNA
-
 ?
5.6.2.1 supercoiled DNA specific for negatively supercoiled DNA, no activity with positively supercoiled DNA Geobacillus stearothermophilus relaxed closed circular DNA
-
 ?
5.6.2.1 supercoiled DNA cleavage sites on pNSI DNA Geobacillus stearothermophilus relaxed closed circular DNA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.2.1 65 70
-
 Geobacillus stearothermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.6.2.1 55 75 55°C: about 90% of maximal activity, 75°C: about 60% of maximal activity. No activity below 37°C Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.6.2.1 65
-
 30 min, 10 mM Tris-HCl buffer, pH 7.5, 1 mM EDTA, 5 mM 2-mercaptoethanol, 0.5 mM PMSF, 10% glycerol, 150 mM NaCl, protein concentration 1.5 mg/ml, enzyme forms MW 80000, MW 100000 and MW 110000, stable Geobacillus stearothermophilus
5.6.2.1 75
-
 30 min, 10 mM Tris-HCl buffer, pH 7.5, 1 mM EDTA, 5 mM 2-mercaptoethanol, 0.5 mM PMSF, 10% glycerol, 150 mM NaCl, protein concentration 1.5 mg/ml, enzyme forms MW 80000, MW 100000 and MW 110000, 30% loss of activity Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.1 7.5 8.5
-
 Geobacillus stearothermophilus