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Literature summary extracted from

  • Dehaye, L.; Alban, C.; Job, C.; Douce, R.; Job, D.
    Kinetics of the two forms of acetyl-CoA carboxylase from Pisum sativum. Correlation of the substrate specificity of the enzymes and sensitivity towards aryloxyphenoxypropionate herbicides (1994), Eur. J. Biochem., 225, 1113-1123.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.2 Quizalofop seed enzyme which carboxylates acety-CoA and propionyl-CoA has two separate active sites. One site binds either acetyl-CoA or propionyl-CoA and is inhibited by quizalofop. The other site is specific for acetyl-CoA and is much less inhibited by quizalofop. The leaf enzyme is not able to carboxylate propionyl-CoA and is insensitive to quizalofop Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 0.038
-
ATP propionyl-CoA Pisum sativum
6.4.1.2 0.226
-
acetyl-CoA
-
Pisum sativum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 220000
-
2 * 220000, SDS-PAGE Pisum sativum
6.4.1.2 480000
-
seed enzyme, gel filtration Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2
-
Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.4.1.2 seed
-
Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.2 0.092
-
seed Pisum sativum
6.4.1.2 0.121
-
leaf Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
Pisum sativum ADP + phosphate + malonyl-CoA
-
?
6.4.1.2 ATP + propionyl-CoA + HCO3- maximal rate is 20% of that for acetyl-CoA Pisum sativum ADP + phosphate + methylmalonyl-CoA
-
?

Subunits

EC Number Subunits Comment Organism
6.4.1.2 dimer 2 * 220000, SDS-PAGE Pisum sativum