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Literature summary extracted from

  • Roessler, P.G.
    Purification and characterization of acetyl-CoA carboxylase from diatom Cyclotella cryptica (1990), Plant Physiol., 92, 73-78.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.4.1.2 citrate no effect Cyclotella cryptica

General Stability

EC Number General Stability Organism
6.4.1.2 citrate, slightly stabilizes Cyclotella cryptica
6.4.1.2 NaCl or KCl required for stability Cyclotella cryptica
6.4.1.2 sulfhydryl reducing agent, e.g. DTT required for stability Cyclotella cryptica

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.4.1.2 ADP
-
 Cyclotella cryptica
6.4.1.2 malonyl-CoA
-
 Cyclotella cryptica
6.4.1.2 palmitate
-
 Cyclotella cryptica
6.4.1.2 palmitoyl-CoA
-
 Cyclotella cryptica
6.4.1.2 phosphate
-
 Cyclotella cryptica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.4.1.2 0.065
-
 ATP
-
 Cyclotella cryptica
6.4.1.2 0.233
-
 acetyl-CoA
-
 Cyclotella cryptica
6.4.1.2 0.75
-
 HCO3-
-
 Cyclotella cryptica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.4.1.2 Mg2+ maximal activity at 2 mM Mg2+ in presence of 1 mM ATP Cyclotella cryptica
6.4.1.2 Mn2+ 20% of activation relative to Mg2+, at 2 mM Cyclotella cryptica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.2 185000
-
 4 * 185000, SDS-PAGE Cyclotella cryptica
6.4.1.2 740000
-
 gel filtration Cyclotella cryptica

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.2 Cyclotella cryptica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.2
-
 Cyclotella cryptica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.4.1.2 14.6
-
-
 Cyclotella cryptica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.2 ATP + acetyl-CoA + HCO3-
-
 Cyclotella cryptica ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + acetyl-CoA + hydrogencarbonate ATP in form of MgATP2- Cyclotella cryptica ADP + phosphate + malonyl-CoA
-
 ?
6.4.1.2 ATP + propionyl-CoA + HCO3- 10% of the activity relative to acetyl-CoA Cyclotella cryptica ADP + phosphate + methylmalonyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.4.1.2 tetramer 4 * 185000, SDS-PAGE Cyclotella cryptica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4.1.2 8.2
-
-
 Cyclotella cryptica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.4.1.2 5.5
-
 4°C, 96% loss of activiyt after 48 h Cyclotella cryptica
6.4.1.2 6.5
-
 maximal stability. 4°C, 3% loss of activity after 48 h Cyclotella cryptica
6.4.1.2 7.5
-
 4°C, 85% loss of activity after 48 h Cyclotella cryptica