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Literature summary extracted from

  • Vollmer, M.D.; Schlömann, M.
    Conversion of 2-chloro-cis,cis-muconate and its metabolites 2-chloro- and 5-chloromuconolactone by chloromuconate cycloisomerases of pJP4 and pAC27 (1995), J. Bacteriol., 177, 2938-2941.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.7 0.0182
-
 2,4-dichloro-cis,cis-muconate
-
 Cupriavidus necator
5.5.1.7 0.0781
-
 2-chloro-cis,cis-muconate measurement of substrate consumption Cupriavidus necator
5.5.1.7 0.0928
-
 cis,cis-muconate 3-chloro-cis,cis-muconate Cupriavidus necator
5.5.1.7 0.274
-
 (+)-2-Chloromuconolactone measurement of substrate consumption Cupriavidus necator
5.5.1.7 0.287
-
 2-chloro-cis,cis-muconate measurement of product formation Cupriavidus necator
5.5.1.7 0.805
-
 (+)-5-chloromuconolactone
-
 Cupriavidus necator
5.5.1.7 0.819
-
 (+)-2-Chloromuconolactone measurement of product formation Cupriavidus necator

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.7 39000
-
 x * 39000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.7 Cupriavidus necator
-
 basonym Alcaligenes eutrophus
-
5.5.1.7 Cupriavidus necator
-
 JMP134, pJP4-encoded enzyme
-
5.5.1.7 Escherichia coli
-
 DH1(pDC100), pAC27-encoded enzyme
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5.1.7 additional information
-
-
 Escherichia coli
5.5.1.7 additional information
-
-
 Cupriavidus necator

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.7 (+)-2-Chloromuconolactone
-
 Escherichia coli trans-4-Carboxymethylenebut-2-en-4-olide + Cl-
-
 ?
5.5.1.7 (+)-2-Chloromuconolactone
-
 Cupriavidus necator trans-4-Carboxymethylenebut-2-en-4-olide + Cl-
-
 ?
5.5.1.7 (+)-5-Chloromuconolactone
-
 Escherichia coli trans-4-Carboxymethylenebut-2-en-4-olide + Cl-
-
 ?
5.5.1.7 (+)-5-Chloromuconolactone
-
 Cupriavidus necator trans-4-Carboxymethylenebut-2-en-4-olide + Cl-
-
 ?
5.5.1.7 2,4-dichloro-cis,cis-muconate
-
 Escherichia coli ?
-
 ?
5.5.1.7 2,4-dichloro-cis,cis-muconate
-
 Cupriavidus necator ?
-
 ?
5.5.1.7 2-Chloro-cis,cis-muconate
-
 Escherichia coli 4-Carboxymethyl-2-chloro-but-2-en-4-olide trans-4-carboxymethylenebut-2-en-4-olide is produced after dehalogenation ?
5.5.1.7 2-Chloro-cis,cis-muconate
-
 Cupriavidus necator 4-Carboxymethyl-2-chloro-but-2-en-4-olide trans-4-carboxymethylenebut-2-en-4-olide is produced after dehalogenation ?
5.5.1.7 3-Chloro-cis,cis-muconate
-
 Escherichia coli cis-4-Carboxymethylenebut-2-en-4-olide
-
 ?
5.5.1.7 3-Chloro-cis,cis-muconate
-
 Cupriavidus necator cis-4-Carboxymethylenebut-2-en-4-olide
-
 ?
5.5.1.7 cis,cis-Muconate
-
 Cupriavidus necator ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.5.1.7 ? x * 39000, SDS-PAGE Escherichia coli
5.5.1.7 ? x * 38000-40000, SDS-PAGE Cupriavidus necator

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.7 2.12
-
 (+)-2-Chloromuconolactone measurement of substrate consumption Cupriavidus necator
5.5.1.7 3.83
-
 2-chloro-cis,cis-muconate measurement of substrate consumption Cupriavidus necator
5.5.1.7 3.95
-
 (+)-2-Chloromuconolactone measurement of product formation Cupriavidus necator
5.5.1.7 10.3
-
 2-chloro-cis,cis-muconate measurement of product formation Cupriavidus necator
5.5.1.7 24.7
-
 (+)-5-chloromuconolactone
-
 Cupriavidus necator
5.5.1.7 44.7
-
 2,4-dichloro-cis,cis-muconate
-
 Cupriavidus necator
5.5.1.7 57.7
-
 3-chloro-cis,cis-muconate
-
 Cupriavidus necator