BRENDA - Enzyme Database

Acetyl-CoA carboxylase from rat liver. EC 6.4.1.2 Acetyl-CoA:carbon-dioxide ligase (ADP-forming)

Tanabe, T.; Nakanishi, S.; Hashimoto, T.; Ogiwara, H.; Nikawa, J.I.; Numa, S.; Methods Enzymol. 71, 5-16 (1981)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
6.4.1.2
citrate
nearly absolute requirement for tricarboxylate activators such as citrate or isocitrate
Rattus norvegicus
6.4.1.2
isocitrate
nearly absolute requirement for tricarboxylate activators such as citrate or isocitrate
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.2
2-(4-Chlorophenoxy)-2-methylpropionate
-
Rattus norvegicus
6.4.1.2
2-Methyl-2-[p-(1,2,3,4-tetrahydro-1-naphthyl)-phenoxy]propionic acid
-
Rattus norvegicus
6.4.1.2
Arachidonoyl-CoA
-
Rattus norvegicus
6.4.1.2
arachidoyl-CoA
-
Rattus norvegicus
6.4.1.2
docosanoyl-CoA
-
Rattus norvegicus
6.4.1.2
Kynurenate
-
Rattus norvegicus
6.4.1.2
lignoceroyl-CoA
-
Rattus norvegicus
6.4.1.2
linolenoyl-CoA
-
Rattus norvegicus
6.4.1.2
linoleoyl-CoA
-
Rattus norvegicus
6.4.1.2
long-chain acyl-CoA thioesters
-
Rattus norvegicus
6.4.1.2
myristoyl-CoA
-
Rattus norvegicus
6.4.1.2
oleoyl-CoA
-
Rattus norvegicus
6.4.1.2
palmitoleoyl-CoA
-
Rattus norvegicus
6.4.1.2
palmitoyl-1,N6-etheno-CoA
-
Rattus norvegicus
6.4.1.2
Palmitoyl-4'-phosphopantetheine
-
Rattus norvegicus
6.4.1.2
palmitoyl-CoA
-
Rattus norvegicus
6.4.1.2
palmitoyl-dephospho-CoA
-
Rattus norvegicus
6.4.1.2
Palmitoyl-inosine-CoA
-
Rattus norvegicus
6.4.1.2
Palmitoyl-keto-CoA
-
Rattus norvegicus
6.4.1.2
S-cetyl-CoA
-
Rattus norvegicus
6.4.1.2
stearoyl-CoA
-
Rattus norvegicus
6.4.1.2
Xanthrenate
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.2
0.015
-
ATP
-
Rattus norvegicus
6.4.1.2
0.016
-
malonyl-CoA
-
Rattus norvegicus
6.4.1.2
0.025
-
acetyl-CoA
-
Rattus norvegicus
6.4.1.2
1.25
-
acetodethio-CoA
-
Rattus norvegicus
6.4.1.2
2.5
-
HCO3-
hindlimb muscle
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.4.1.2
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
6.4.1.2
phosphate
contains 2.1 mol phosphate per 215000 g of protein
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.2
230000
-
x * 230000, SDS-PAGE
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.4.1.2
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.4.1.2
-
Rattus norvegicus
Reaction
EC Number
Reaction
Commentary
Organism
6.4.1.2
ATP + acetyl-CoA + hydrogencarbonate = ADP + phosphate + malonyl-CoA
ordered bi bi uni uni ping-pong mechanism
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
6.4.1.2
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4.1.2
6.05
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.4.1.2
ATP + acetodethio-CoA + HCO3-
-
37586
Rattus norvegicus
?
-
-
-
-
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
37586
Rattus norvegicus
ADP + phosphate + malonyl-CoA
-
37586
Rattus norvegicus
-
Subunits
EC Number
Subunits
Commentary
Organism
6.4.1.2
?
x * 230000, SDS-PAGE
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.4.1.2
biotin
contains 1 mol of biotin per 237000 g of enzyme
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
6.4.1.2
citrate
nearly absolute requirement for tricarboxylate activators such as citrate or isocitrate
Rattus norvegicus
6.4.1.2
isocitrate
nearly absolute requirement for tricarboxylate activators such as citrate or isocitrate
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.4.1.2
biotin
contains 1 mol of biotin per 237000 g of enzyme
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.4.1.2
2-(4-Chlorophenoxy)-2-methylpropionate
-
Rattus norvegicus
6.4.1.2
2-Methyl-2-[p-(1,2,3,4-tetrahydro-1-naphthyl)-phenoxy]propionic acid
-
Rattus norvegicus
6.4.1.2
Arachidonoyl-CoA
-
Rattus norvegicus
6.4.1.2
arachidoyl-CoA
-
Rattus norvegicus
6.4.1.2
docosanoyl-CoA
-
Rattus norvegicus
6.4.1.2
Kynurenate
-
Rattus norvegicus
6.4.1.2
lignoceroyl-CoA
-
Rattus norvegicus
6.4.1.2
linolenoyl-CoA
-
Rattus norvegicus
6.4.1.2
linoleoyl-CoA
-
Rattus norvegicus
6.4.1.2
long-chain acyl-CoA thioesters
-
Rattus norvegicus
6.4.1.2
myristoyl-CoA
-
Rattus norvegicus
6.4.1.2
oleoyl-CoA
-
Rattus norvegicus
6.4.1.2
palmitoleoyl-CoA
-
Rattus norvegicus
6.4.1.2
palmitoyl-1,N6-etheno-CoA
-
Rattus norvegicus
6.4.1.2
Palmitoyl-4'-phosphopantetheine
-
Rattus norvegicus
6.4.1.2
palmitoyl-CoA
-
Rattus norvegicus
6.4.1.2
palmitoyl-dephospho-CoA
-
Rattus norvegicus
6.4.1.2
Palmitoyl-inosine-CoA
-
Rattus norvegicus
6.4.1.2
Palmitoyl-keto-CoA
-
Rattus norvegicus
6.4.1.2
S-cetyl-CoA
-
Rattus norvegicus
6.4.1.2
stearoyl-CoA
-
Rattus norvegicus
6.4.1.2
Xanthrenate
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.4.1.2
0.015
-
ATP
-
Rattus norvegicus
6.4.1.2
0.016
-
malonyl-CoA
-
Rattus norvegicus
6.4.1.2
0.025
-
acetyl-CoA
-
Rattus norvegicus
6.4.1.2
1.25
-
acetodethio-CoA
-
Rattus norvegicus
6.4.1.2
2.5
-
HCO3-
hindlimb muscle
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
6.4.1.2
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
6.4.1.2
phosphate
contains 2.1 mol phosphate per 215000 g of protein
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
6.4.1.2
230000
-
x * 230000, SDS-PAGE
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.4.1.2
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
6.4.1.2
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.4.1.2
6.05
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.4.1.2
ATP + acetodethio-CoA + HCO3-
-
37586
Rattus norvegicus
?
-
-
-
-
6.4.1.2
ATP + acetyl-CoA + HCO3-
-
37586
Rattus norvegicus
ADP + phosphate + malonyl-CoA
-
37586
Rattus norvegicus
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
6.4.1.2
?
x * 230000, SDS-PAGE
Rattus norvegicus