EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.3.1.2 | L-methionine sulfoximine | - |
Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.3.1.2 | 2.7 | - |
Glu | - |
Mycobacterium tuberculosis | |
6.3.1.2 | 2.9 | - |
ATP | Gln | Mycobacterium tuberculosis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
6.3.1.2 | extracellular | 30% of the total measurable enzyme activity is released into the culture medium | Mycobacterium tuberculosis | - |
- |
6.3.1.2 | phagosome | Mycobacterium tuberculosis releases the enzyme into its phagosome in infected human monocytes | Mycobacterium tuberculosis | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 58000 | - |
12 * 58000, arranged in two superimpose hexagons, SDS-PAGE | Mycobacterium tuberculosis |
6.3.1.2 | 680000 | - |
gel filtration | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + L-Glu + NH4+ | Mycobacterium tuberculosis | roles of the enzyme in pathogenesis of Mycobacterium tuberculosis infection: 1. synthesis of Glu, that is a major component of the cell wall of pathogenic mycobacteria, 2. modulation of the NH4+ level in the Mycobacterium tuberculosis phagosome | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.3.1.2 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | additional information | - |
- |
Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.3.1.2 | ATP + L-Glu + NH4+ | - |
Mycobacterium tuberculosis | ADP + phosphate + L-Gln | - |
? | |
6.3.1.2 | ATP + L-Glu + NH4+ | roles of the enzyme in pathogenesis of Mycobacterium tuberculosis infection: 1. synthesis of Glu, that is a major component of the cell wall of pathogenic mycobacteria, 2. modulation of the NH4+ level in the Mycobacterium tuberculosis phagosome | Mycobacterium tuberculosis | ? | - |
? | |
6.3.1.2 | GTP + L-Glu + NH4+ | 45% of the activity relative to ATP | Mycobacterium tuberculosis | GDP + phosphate + L-Gln | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.3.1.2 | dodecamer | 12 * 58000, arranged in two superimpose hexagons, SDS-PAGE | Mycobacterium tuberculosis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 20 | - |
pH 7.0, 10 mM EDTA, 10 mM imidazole, complete loss of activity after 20 min | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
6.3.1.2 | 7 | - |
in presence of Mn2+ | Mycobacterium tuberculosis |
6.3.1.2 | 7.5 | - |
in presence of Mg2+ or Co2+ | Mycobacterium tuberculosis |