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Literature summary extracted from

  • Kumar, S.; Nicholas, D.J.D.
    Purification, properties and regulation of glutamine synthetase from Nitrobacter agilis (1984), J. Gen. Microbiol., 130, 959-966.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 Ala
-
 Nitrobacter winogradskyi
6.3.1.2 Gly
-
 Nitrobacter winogradskyi
6.3.1.2 NH4Cl
-
 Nitrobacter winogradskyi
6.3.1.2 Ser
-
 Nitrobacter winogradskyi
6.3.1.2 Trp
-
 Nitrobacter winogradskyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 0.2
-
 NH4+ biosynthetic assay Nitrobacter winogradskyi
6.3.1.2 2.6
-
 hydroxylamine glutamyltransferase reaction Nitrobacter winogradskyi
6.3.1.2 6.3
-
 Glu biosynthetic assay Nitrobacter winogradskyi
6.3.1.2 11.6
-
 Gln glutamyl transferase reaction Nitrobacter winogradskyi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Co2+ divalent cation required, decreasing order of effectiveness at 10 mM in transferase activity: Mn2+, Cu2+, Mg2+, Co2+. For biosynthetic activity: Mg2+, Mn2+, Zn2+/Cu2+, Ni2+ Nitrobacter winogradskyi
6.3.1.2 Cu2+ divalent cation required, decreasing order of effectiveness at 10 mM in transferase activity: Mn2+, Cu2+, Mg2+, Co2+. For biosynthetic activity: Mg2+, Mn2+, Zn2+/Cu2+, Ni2+ Nitrobacter winogradskyi
6.3.1.2 Mg2+ divalent cation required, order of effectiveness at 10 mM in transferase activity: Mn2+, Cu2+, Mg2+, Co2+. For biosynthetic activity: Mg2+, Mn2+, Zn2+, Cu2+, Ni2+ Nitrobacter winogradskyi
6.3.1.2 Mn2+ divalent cation required, order of effectiveness at 10 mM in transferase activity: Mn2+, Cu2+, Mg2+, Co2+. For biosynthetic activity: Mg2+, Mn2+, Zn2+, Cu2+, Ni2+ Nitrobacter winogradskyi
6.3.1.2 Ni2+ divalent cation required, decreasing order of effectiveness at 10 mM in transferase activity: Mn2+, Cu2+, Mg2+, Co2+. For biosynthetic activity: Mg2+, Mn2+, Zn2+/Cu2+, Ni2+ Nitrobacter winogradskyi
6.3.1.2 Zn2+ divalent cation required, decreasing order of effectiveness at 10 mM in transferase activity: Mn2+, Cu2+, Mg2+, Co2+. For biosynthetic activity: Mg2+, Mn2+, Zn2+/Cu2+, Ni2+ Nitrobacter winogradskyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 58000
-
 12 * 58000, SDS-PAGE Nitrobacter winogradskyi
6.3.1.2 700000
-
 gel filtration Nitrobacter winogradskyi

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Nitrobacter winogradskyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2
-
 Nitrobacter winogradskyi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 220
-
-
 Nitrobacter winogradskyi

Storage Stability

EC Number Storage Stability Organism
6.3.1.2 -10°C, stable for several weeks Nitrobacter winogradskyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-Glu + NH4+
-
 Nitrobacter winogradskyi ADP + phosphate + L-Gln
-
 ?
6.3.1.2 Gln + hydroxylamine + ADP
-
 Nitrobacter winogradskyi gamma-Glutamylhydroxamate + NH4+ + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.1.2 dodecamer 12 * 58000, SDS-PAGE Nitrobacter winogradskyi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 7
-
-
 Nitrobacter winogradskyi
6.3.1.2 7.8
-
 deadenylylated form Nitrobacter winogradskyi