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Literature summary extracted from

  • Guo, W.; Gonzalez-Candelas, L.; Kolattukudy, P.E.
    Cloning of a novel constitutively expressed pectate lyase gene pelB from Fusarium solani f. sp. pisi (Nectria haematococca, mating type VI) and characterization of the gene product expressed in Pichia pastoris (1995), J. Bacteriol., 177, 7070-7077.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.2 pectate lyase B, expression in Pichia pastoris Fusarium solani

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 EGTA
-
 Fusarium solani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.2 additional information
-
 additional information
-
 Fusarium solani

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ required Fusarium solani
4.2.2.2 Mn2+ 11% of the activation with Ca2+ Fusarium solani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.2 additional information Fusarium solani constitutive enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Fusarium solani
-
 pectate lyase B
-
4.2.2.2 Fusarium solani
-
 f. sp. pisi
-
4.2.2.2 Fusarium solani
-
 Nectria haematococca mating type VI
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.2.2 side-chain modification glycoprotein Fusarium solani

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2 pectate lyase B Fusarium solani
4.2.2.2 recombinant enzyme Fusarium solani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 additional information constitutive enzyme Fusarium solani ?
-
 ?
4.2.2.2 pectin about 70% methylated Fusarium solani ?
-
 ?
4.2.2.2 polygalacturonic acid
-
 Fusarium solani unsaturated oligogalacturonate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 10
-
-
 Fusarium solani