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Literature summary extracted from

  • Kozianowsky, G.; Canganella, F.; Rainey, F.A.; Hippe, H.; Antranikian, G.
    Purification and characterization of thermostable pectate-lyases from a newly isolated thermophilic bacterium, Thermoanaerobacter italicus sp. nov (1997), Extremophiles, 1, 171-182.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 EDTA
-
 Thermoanaerobacter italicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.2 additional information
-
 additional information Km for citrus pectin: 5.0 g/l Thermoanaerobacter italicus
4.2.2.2 additional information
-
 additional information Km for pectate: 0.5 g/l Thermoanaerobacter italicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ stimulates Thermoanaerobacter italicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 additional information
-
 pectate lyase b shows two bands in non-denaturing PAGE: 200000 Da and 252000 Da Thermoanaerobacter italicus
4.2.2.2 93000
-
 1 * 93000 + 1 * 158000, pectate lyase b, SDS-PAGE Thermoanaerobacter italicus
4.2.2.2 135000
-
 1 * 135000, pectate lyase a, SDS-PAGE Thermoanaerobacter italicus
4.2.2.2 148000
-
 pectate lyase a, non-denaturing PAGE Thermoanaerobacter italicus
4.2.2.2 158000
-
 1 * 93000 + 1 * 158000, pectate lyase b, SDS-PAGE Thermoanaerobacter italicus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Thermoanaerobacter italicus
-
 nov., pectate lyase a and pectate lyase b
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2 pectate lyase a and pectate lyase b Thermoanaerobacter italicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 pectate
-
 Thermoanaerobacter italicus ?
-
 ?
4.2.2.2 pectin
-
 Thermoanaerobacter italicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 dimer 1 * 93000 + 1 * 158000, pectate lyase b, SDS-PAGE Thermoanaerobacter italicus
4.2.2.2 monomer 1 * 135000, pectate lyase a, SDS-PAGE Thermoanaerobacter italicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 75
-
 pectate lyase a Thermoanaerobacter italicus
4.2.2.2 80
-
 pectate lyase b Thermoanaerobacter italicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.2 60 85 60°C: about 55% of maximal activity, 80°C: about 90% of maximal activity, 85°C: about 20% of maximal activity, pectate lyase a Thermoanaerobacter italicus
4.2.2.2 60 90 60°C: about 45% of maximal activity, 90°C: about 30% of maximal activity, pectate lyase b Thermoanaerobacter italicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 70
-
 2 h, about 10% loss of activity Thermoanaerobacter italicus
4.2.2.2 80
-
 half-life: about 60 min Thermoanaerobacter italicus
4.2.2.2 90
-
 complete loss of activity after 5 min Thermoanaerobacter italicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 8
-
 pectate lyase a Thermoanaerobacter italicus
4.2.2.2 9
-
 pectate lyase B Thermoanaerobacter italicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.2 6 10 pH 6.0: about 40% of maximal activity, pH 10.0: about 50% of maximal activity, pectate lyase a Thermoanaerobacter italicus
4.2.2.2 7 10 pH 7.0: about 35% of maximal activity, pH 10.0: 50% of maximal activity, pectate lyase b Thermoanaerobacter italicus