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Literature summary extracted from

  • Sato, M.; Kaji, A.
    Another pectate lyase produced by Streptomyces nitrosporeus (1980), Agric. Biol. Chem., 44, 1345-1349.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 EDTA
-
 Streptomyces nitrosporeus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ required Streptomyces nitrosporeus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 41000
-
 x * 41000, SDS-PAGE Streptomyces nitrosporeus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Streptomyces nitrosporeus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2
-
 Streptomyces nitrosporeus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.2 319
-
-
 Streptomyces nitrosporeus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 hexagalacturonic acid 140% of the activity with low molecular weight polygalacturonic acid Streptomyces nitrosporeus ?
-
 ?
4.2.2.2 additional information macerating activity on potato tissue and Ganpi bark Streptomyces nitrosporeus ?
-
 ?
4.2.2.2 pentagalacturonic acid 215% of the activity with low molecular weight polygalacturonic acid Streptomyces nitrosporeus saturated digalacturonic acid + unsaturated trigalacturonic acid + saturated trigalacturonic acid + unsaturated digalacturonic acid
-
 ?
4.2.2.2 polygalacturonic acid random attack Streptomyces nitrosporeus unsaturated oligogalacturonate higher oligomers are detected in the early stage of degradation, unsaturated monogalacturonic acids and digalacturonic acids are also detected after prolonged degradation ?
4.2.2.2 polygalacturonic acid low molecular weight. High molecular weight polygalacturonic acid is attacked at 46% of the activity with low molecular weight polygalacturonic acid Streptomyces nitrosporeus unsaturated oligogalacturonate higher oligomers are detected in the early stage of degradation, unsaturated monogalacturonic acids and digalacturonic acids are also detected after prolonged degradation ?
4.2.2.2 tetragalacturonic acid 141% of the activity with low molecular weight polygalacturonic acid Streptomyces nitrosporeus altered trigalacturonic acid + digalacturonic acid + altered digalacturionic acid + D-galacturonic acid
-
 ?
4.2.2.2 trigalacturonic acid 3% of the activity with low molecular weight polygalacturonic acid Streptomyces nitrosporeus altered digalacturonic acid + galacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 41000, SDS-PAGE Streptomyces nitrosporeus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 50
-
 in a 2 min reaction at pH 9.5 Streptomyces nitrosporeus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 40
-
 pH 7.0, 10 min, stable below Streptomyces nitrosporeus
4.2.2.2 50
-
 10 min, pH 7.0, 35% loss of activity Streptomyces nitrosporeus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 10
-
-
 Streptomyces nitrosporeus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.2 4 11 2°C, 48 h, stable Streptomyces nitrosporeus