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Literature summary extracted from

  • Kawasaki, K.; Yokota, A.; Tomita, F.
    Enzymatic synthesis of L-tryptophan by Enterobacter aerogenes tryptophanase highly expressed in Escherichia coli, and some properties of the purified enzyme (1995), Biosci. Biotechnol. Biochem., 59, 1938-1943.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.1 expression in Escherichia coli Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.1 210000
-
 gel filtration Klebsiella aerogenes

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.1 Escherichia coli
-
-
-
4.1.99.1 Klebsiella aerogenes
-
 SM-18
-
4.1.99.1 Klebsiella aerogenes SM-18
-
 SM-18
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.1
-
 Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.99.1 16.7
-
-
 Klebsiella aerogenes
4.1.99.1 23.5
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.1 L-Trp + H2O
-
 Escherichia coli indole + pyruvate + NH4+
-
 ?
4.1.99.1 L-Trp + H2O
-
 Klebsiella aerogenes indole + pyruvate + NH4+
-
 ?
4.1.99.1 L-Trp + H2O
-
 Klebsiella aerogenes SM-18 indole + pyruvate + NH4+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.99.1 55
-
 10 min, stable up to Escherichia coli
4.1.99.1 60
-
-
 Escherichia coli
4.1.99.1 60
-
 10 min, stable up to Klebsiella aerogenes