EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.99.1 | K+ | promotes the conversion of the inactive holoenzyme into the active holoenzyme rather than being involved in the conversion of the apoenzyme and pyridoxal 5'-phosphate into the active holoenzyme | Escherichia coli | |
4.1.99.1 | NH4+ | promotes the conversion of the inactive holoenzyme into the active holoenzyme rather than being involved in the conversion of the apoenzyme and pyridoxal 5'-phosphate into the active holoenzyme | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.99.1 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.1 | L-Trp + H2O | - |
Escherichia coli | indole + pyruvate + NH4+ | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.99.1 | 5 | - |
stable in presence of K+, gradual loss of activity in absence of K+ | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.99.1 | pyridoxal 5'-phosphate | dependent on | Escherichia coli | |
4.1.99.1 | pyridoxal 5'-phosphate | conversion of apoenzyme into the active holoenzyme is attained at 30°C in Tris-HCl buffer, pH 8.0, containing pyridoxal 5'-phosphate and K+, no conversion occurs at 5°C | Escherichia coli |