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Literature summary extracted from

  • Behbahani-Nejad, I.; Dye, J.L.; Suelter, C.H.
    Tryptophanase from Escherichia coli B/1t7-A (1987), Methods Enzymol., 142, 414-422.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.99.1 0.019
-
 indole
-
 Escherichia coli
4.1.99.1 0.06
-
 S-o-nitrophenyl-L-Cys
-
 Escherichia coli
4.1.99.1 0.067
-
 S-benzylcysteine
-
 Escherichia coli
4.1.99.1 0.33
-
 L-Trp
-
 Escherichia coli
4.1.99.1 1.2
-
 beta-chloroalanine
-
 Escherichia coli
4.1.99.1 11
-
 Cys
-
 Escherichia coli
4.1.99.1 12
-
 S-methyl-L-Cys
-
 Escherichia coli
4.1.99.1 70
-
 pyruvate
-
 Escherichia coli
4.1.99.1 160
-
 L-Ser strain B/1t7-A, 37°C Escherichia coli
4.1.99.1 345
-
 NH4+
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.99.1 K+ monovalent cation activator required Escherichia coli
4.1.99.1 NH4+ monovalent cation activator required Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.99.1 220000
-
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.1 Escherichia coli
-
-
-
4.1.99.1 Escherichia coli B/1t7-A
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.1
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.99.1 50
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.1.99.1 stored as a suspension under nitrogen in 0.1 M potassium phosphate, pH 8.0, 0.2 mM dithiothreitol, 1 mM EDTA, and 2 M (NH4)2SO4 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.1 beta-chloroalanine + H2O
-
 Escherichia coli ?
-
 ?
4.1.99.1 beta-chloroalanine + H2O
-
 Escherichia coli B/1t7-A ?
-
 ?
4.1.99.1 Cys + H2O
-
 Escherichia coli ?
-
 ?
4.1.99.1 L-Trp + H2O
-
 Escherichia coli indole + pyruvate + NH4+
-
 ?
4.1.99.1 S-benzylcysteine + H2O
-
 Escherichia coli ?
-
 ?
4.1.99.1 S-benzylcysteine + H2O
-
 Escherichia coli B/1t7-A ?
-
 ?
4.1.99.1 S-methyl-L-Cys + indole
-
 Escherichia coli Trp + ?
-
 ?
4.1.99.1 S-methyl-L-Cys + indole
-
 Escherichia coli B/1t7-A Trp + ?
-
 ?
4.1.99.1 S-o-nitrophenyl-L-Cys + H2O
-
 Escherichia coli o-nitrothiophenol + pyruvate + NH3
-
 ?
4.1.99.1 S-o-nitrophenyl-L-Cys + H2O
-
 Escherichia coli B/1t7-A o-nitrothiophenol + pyruvate + NH3
-
 ?
4.1.99.1 Ser + indole
-
 Escherichia coli Trp + ?
-
 ?
4.1.99.1 Ser + indole
-
 Escherichia coli B/1t7-A Trp + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.99.1 tetramer 4 * ? Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.1 pyridoxal 5'-phosphate dependent on Escherichia coli
4.1.99.1 pyridoxal 5'-phosphate binds 1 mol of pyridoxal 5'-phosphate per subunit Escherichia coli