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Literature summary extracted from

  • Yu, M.S.; Lee, C.Y.
    Expression and characterization of the prtV gene encoding a collagenase from Vibrio parahaemolyticus in Escherichia coli (1999), Microbiology, 145, 143-150.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.3 expressed in Escherichia coli Vibrio parahaemolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.3 1,10-phenanthroline activity restored by Co2+, Mn2+, Ca2+, Cu2+, Ni2+ and Zn2+ Vibrio parahaemolyticus
3.4.24.3 2-mercaptoethanol
-
 Vibrio parahaemolyticus
3.4.24.3 EDTA activity restored by Co2+, Mn2+, Ca2+, Cu2+, Ni2+ and Zn2+ Vibrio parahaemolyticus
3.4.24.3 EGTA activity restored by Co2+, Mn2+, Ca2+, Cu2+, Ni2+ and Zn2+ Vibrio parahaemolyticus
3.4.24.3 Guanidine-HCl
-
 Vibrio parahaemolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.3 additional information
-
 additional information
-
 Vibrio parahaemolyticus
3.4.24.3 1.06
-
 2-furanacryloyl-Leu-Gly-Pro-Ala
-
 Vibrio parahaemolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.3 62000
-
 gel filtration Vibrio parahaemolyticus
3.4.24.3 64000
-
 1 * 64000, SDS-PAGE Vibrio parahaemolyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.3 Collagen + H2O Vibrio parahaemolyticus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.3 Vibrio parahaemolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.3 expressed in Escherichia coli Vibrio parahaemolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.3 2-furanacryloyl-Leu-Gly-Pro-Ala + H2O
-
 Vibrio parahaemolyticus 2-furanacryloyl-Leu + Gly-Pro-Ala
-
 ?
3.4.24.3 Collagen + H2O
-
 Vibrio parahaemolyticus Hydrolyzed collagen
-
 ?
3.4.24.3 Collagen + H2O
-
 Vibrio parahaemolyticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.3 monomer 1 * 64000, SDS-PAGE Vibrio parahaemolyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.3 40
-
-
 Vibrio parahaemolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.3 7.5
-
-
 Vibrio parahaemolyticus