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Literature summary extracted from

  • Merkel, J.R.; Dreisbach, J.H.
    Purification and characterization of a marine bacterial collagenase (1978), Biochemistry, 17, 2857-2863.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.3 phosphate buffer
-
 Vibrio sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.3 24000
-
 x * 24000 + x * 28000, SDS-PAGE after denaturation with SDS and beta-mercaptoethanol Vibrio sp.
3.4.24.3 28000
-
 x * 24000 + x * 28000, SDS-PAGE after denaturation with SDS and beta-mercaptoethanol Vibrio sp.
3.4.24.3 105000
-
 gel filtration Vibrio sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.3 Collagen + H2O Vibrio sp. degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro ?
-
 ?
3.4.24.3 Collagen + H2O Vibrio sp. B-30 degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.3 Vibrio sp.
-
 B-30
-
3.4.24.3 Vibrio sp. B-30
-
 B-30
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.3 partial Vibrio sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.3 culture medium
-
 Vibrio sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.3 additional information
-
-
 Vibrio sp.

Storage Stability

EC Number Storage Stability Organism
3.4.24.3 4°C, 24 h, pH 4.4-7.4 Vibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.3 Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form Vibrio sp. Hydrolyzed collagen
-
 ?
3.4.24.3 Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form Vibrio sp. B-30 Hydrolyzed collagen
-
 ?
3.4.24.3 Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro Vibrio sp. ?
-
 ?
3.4.24.3 Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro Vibrio sp. B-30 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.3 tetramer x * 24000 + x * 28000, SDS-PAGE after denaturation with SDS and beta-mercaptoethanol Vibrio sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.3 37
-
-
 Vibrio sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.3 7.6
-
-
 Vibrio sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.24.3 4.4 7.4 4°C, 24 h Vibrio sp.