Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Soru, E.; Zaharia, O.; Szabados, J.; Rudescu, K.; Bittner, J.; Ficiu, S.
    Purification by molecular sieve chromatography on polyacrylamide Bio Gel P-300 of Clostridium histolyticum collagenase (1970), Enzymologia, 39, 33-48.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.3 68000
-
 ? * 68000, alpha, SDS-PAGE Hathewaya histolytica
3.4.24.3 79000
-
 ? * 79000, gamma, SDS-PAGE Hathewaya histolytica
3.4.24.3 100000
-
 gamma, SDS-PAGE Hathewaya histolytica
3.4.24.3 110000
-
 ? * 110000, delta, SDS-PAGE Hathewaya histolytica
3.4.24.3 115000
-
 ? * 115000, beta, SDS-PAGE Hathewaya histolytica
3.4.24.3 120000
-
 gel filtration Hathewaya histolytica
3.4.24.3 125000
-
 ? * 125000, zeta, SDS-PAGE Hathewaya histolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.3 Collagen + H2O Hathewaya histolytica degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.3 Hathewaya histolytica
-
 2 fractions differ in specificity
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.3
-
 Hathewaya histolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.3 Azocoll + H2O
-
 Hathewaya histolytica Hydrolyzed azocoll
-
 ?
3.4.24.3 carbobenzoxy-Gly-Pro-Gly-Gly-Pro-Ala + H2O
-
 Hathewaya histolytica carbobenzoxy-Gly-Pro-Gly + Gly-Pro-Ala
-
 ?
3.4.24.3 Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence-Pro-Xaa-Gly-Pro, nondenatured triple helical form Hathewaya histolytica Hydrolyzed collagen
-
 ?
3.4.24.3 Collagen + H2O degradation of helical regions of native collagen to small fragments, preferential cleavage: -Gly in the sequence Pro-Xaa-Gly-Pro Hathewaya histolytica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.3 ? ? * 125000, zeta, SDS-PAGE Hathewaya histolytica
3.4.24.3 ? ? * 68000, alpha, SDS-PAGE Hathewaya histolytica
3.4.24.3 ? ? * 79000, gamma, SDS-PAGE Hathewaya histolytica
3.4.24.3 ? ? * 110000, delta, SDS-PAGE Hathewaya histolytica
3.4.24.3 ? ? * 115000, beta, SDS-PAGE Hathewaya histolytica