BRENDA - Enzyme Database

Endopeptidase 24.15 from rat testes. Isolation of the enzyme and its specificity toward synthetic and natural peptides, including enkephalin-containing peptides

Orlowski, M.; Reznik, S.; Ayala, J.; Pierotti, A.R.; Biochem. J. 261, 951-958 (1989) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.24.15
dithiothreitol
-
Rattus norvegicus
3.4.24.15
EDTA
-
Rattus norvegicus
3.4.24.15
EGTA
-
Rattus norvegicus
3.4.24.15
leupeptin
weak
Rattus norvegicus
3.4.24.15
NEM
-
Rattus norvegicus
3.4.24.15
PCMB
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.24.15
0.037
-
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu
-
Rattus norvegicus
3.4.24.15
0.038
-
Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro
-
Rattus norvegicus
3.4.24.15
0.058
-
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
0.06
-
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile
-
Rattus norvegicus
3.4.24.15
0.067
-
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
Rattus norvegicus
3.4.24.15
0.095
-
pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
-
Rattus norvegicus
3.4.24.15
0.1
-
tert-butoxycarbonyl-Phe-Ala-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
0.39
-
benzoyl-Gly-Phe-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
0.83
-
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
1.36
-
benzoyl-Gly-Gly-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
1.54
-
benzoyl-Gly-Ala-Ala-Leu-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
3.45
-
benzoyl-Gly-Ala-Ala-Gly-p-aminobenzoate
-
Rattus norvegicus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.24.15
Co2+
restores activity after EDTA treatment
Rattus norvegicus
3.4.24.15
Zn2+
restores activity after treatment with EDTA
Rattus norvegicus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.24.15
Rattus norvegicus
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.24.15
testis
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.24.15
20.3
-
-
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.24.15
adrenorphin
-
37059
Rattus norvegicus
peptide fragments
-
-
-
?
3.4.24.15
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
i.e. bradykinin, cleavage site: Phe5-Ser6
37059
Rattus norvegicus
Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg
-
-
-
?
3.4.24.15
benzoyl-Gly-Ala-Ala-Gly + H2O
-
37059
Rattus norvegicus
benzoyl-Gly + Ala-Ala-Gly
-
-
-
?
3.4.24.15
benzoyl-Gly-Ala-Ala-Gly-p-aminobenzoate
-
37059
Rattus norvegicus
benzoyl-Gly + Ala-Ala-Gly-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
benzoyl-Gly-Ala-Ala-Leu-p-aminobenzoate
-
37059
Rattus norvegicus
benzoyl-Gly + Ala-Ala-Leu-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
benzoyl-Gly + Ala-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
benzoyl-Gly-Gly-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
benzoyl-Gly + Gly-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
benzoyl-Gly-Phe-Ala-Ala-Phe-p-aminobenzoate
inactive with benzoyl-Gly-D-Phe-Ala-Ala-Phe-p-aminobenzoate
37059
Rattus norvegicus
benzoyl-Gly-Phe + Ala-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
benzoyl-Gly-Phe-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
benzoyl-Gly + Phe-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
Met-enkephalin-Arg6-Gly7-Leu8
-
37059
Rattus norvegicus
?
-
-
-
?
3.4.24.15
additional information
cleaves preferentially bonds on the carboxyl side of hydrophobic amino acids
37059
Rattus norvegicus
?
-
-
-
?
3.4.24.15
pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 + H2O
i.e. luteinizing hormone-releasing hormone
37059
Rattus norvegicus
pGlu-His-Trp-Ser-Tyr + Gly-Leu-Arg-Pro-Gly-NH2
-
37059
Rattus norvegicus
?
3.4.24.15
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu
i.e. neurotensin
37059
Rattus norvegicus
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg + Arg-Pro-Tyr-Ile-Leu
-
37059
Rattus norvegicus
?
3.4.24.15
tert-butoxycarbonyl-Phe-Ala-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
tert-butoxycarbonyl-Phe + Ala-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile + H2O
i.e. dynorphin A(1-8)
37059
Rattus norvegicus
Tyr-Gly-Gly-Phe-Leu + Arg-Arg-Ile
-
-
-
?
3.4.24.15
Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro
beta-neoendorphin
37059
Rattus norvegicus
Tyr-Gly-Gly-Phe-Leu + Arg-Lys-Tyr-Pro
-
37059
Rattus norvegicus
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.24.15
4.54
-
benzoyl-Gly-Gly-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
5.3
-
benzoyl-Gly-Ala-Ala-Gly
-
Rattus norvegicus
3.4.24.15
7.9
-
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu
-
Rattus norvegicus
3.4.24.15
12
-
pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
-
Rattus norvegicus
3.4.24.15
17
-
benzoyl-Gly-Ala-Ala-Leu-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
19.2
-
benzoyl-Gly-Phe-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
33.8
-
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
Rattus norvegicus
3.4.24.15
37.9
-
Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro
-
Rattus norvegicus
3.4.24.15
48.3
-
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
86.4
-
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile
-
Rattus norvegicus
3.4.24.15
94.1
-
tert-butoxycarbonyl-Phe-Ala-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
179
-
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.24.15
dithiothreitol
-
Rattus norvegicus
3.4.24.15
EDTA
-
Rattus norvegicus
3.4.24.15
EGTA
-
Rattus norvegicus
3.4.24.15
leupeptin
weak
Rattus norvegicus
3.4.24.15
NEM
-
Rattus norvegicus
3.4.24.15
PCMB
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.24.15
0.037
-
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu
-
Rattus norvegicus
3.4.24.15
0.038
-
Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro
-
Rattus norvegicus
3.4.24.15
0.058
-
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
0.06
-
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile
-
Rattus norvegicus
3.4.24.15
0.067
-
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
Rattus norvegicus
3.4.24.15
0.095
-
pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
-
Rattus norvegicus
3.4.24.15
0.1
-
tert-butoxycarbonyl-Phe-Ala-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
0.39
-
benzoyl-Gly-Phe-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
0.83
-
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
1.36
-
benzoyl-Gly-Gly-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
1.54
-
benzoyl-Gly-Ala-Ala-Leu-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
3.45
-
benzoyl-Gly-Ala-Ala-Gly-p-aminobenzoate
-
Rattus norvegicus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.24.15
Co2+
restores activity after EDTA treatment
Rattus norvegicus
3.4.24.15
Zn2+
restores activity after treatment with EDTA
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.24.15
testis
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.24.15
20.3
-
-
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.24.15
adrenorphin
-
37059
Rattus norvegicus
peptide fragments
-
-
-
?
3.4.24.15
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
i.e. bradykinin, cleavage site: Phe5-Ser6
37059
Rattus norvegicus
Arg-Pro-Pro-Gly-Phe + Ser-Pro-Phe-Arg
-
-
-
?
3.4.24.15
benzoyl-Gly-Ala-Ala-Gly + H2O
-
37059
Rattus norvegicus
benzoyl-Gly + Ala-Ala-Gly
-
-
-
?
3.4.24.15
benzoyl-Gly-Ala-Ala-Gly-p-aminobenzoate
-
37059
Rattus norvegicus
benzoyl-Gly + Ala-Ala-Gly-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
benzoyl-Gly-Ala-Ala-Leu-p-aminobenzoate
-
37059
Rattus norvegicus
benzoyl-Gly + Ala-Ala-Leu-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
benzoyl-Gly + Ala-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
benzoyl-Gly-Gly-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
benzoyl-Gly + Gly-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
benzoyl-Gly-Phe-Ala-Ala-Phe-p-aminobenzoate
inactive with benzoyl-Gly-D-Phe-Ala-Ala-Phe-p-aminobenzoate
37059
Rattus norvegicus
benzoyl-Gly-Phe + Ala-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
benzoyl-Gly-Phe-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
benzoyl-Gly + Phe-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
Met-enkephalin-Arg6-Gly7-Leu8
-
37059
Rattus norvegicus
?
-
-
-
?
3.4.24.15
additional information
cleaves preferentially bonds on the carboxyl side of hydrophobic amino acids
37059
Rattus norvegicus
?
-
-
-
?
3.4.24.15
pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2 + H2O
i.e. luteinizing hormone-releasing hormone
37059
Rattus norvegicus
pGlu-His-Trp-Ser-Tyr + Gly-Leu-Arg-Pro-Gly-NH2
-
37059
Rattus norvegicus
?
3.4.24.15
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu
i.e. neurotensin
37059
Rattus norvegicus
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg + Arg-Pro-Tyr-Ile-Leu
-
37059
Rattus norvegicus
?
3.4.24.15
tert-butoxycarbonyl-Phe-Ala-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
tert-butoxycarbonyl-Phe + Ala-Ala-Phe-p-aminobenzoate
-
37059
Rattus norvegicus
?
3.4.24.15
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile + H2O
i.e. dynorphin A(1-8)
37059
Rattus norvegicus
Tyr-Gly-Gly-Phe-Leu + Arg-Arg-Ile
-
-
-
?
3.4.24.15
Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro
beta-neoendorphin
37059
Rattus norvegicus
Tyr-Gly-Gly-Phe-Leu + Arg-Lys-Tyr-Pro
-
37059
Rattus norvegicus
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.24.15
4.54
-
benzoyl-Gly-Gly-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
5.3
-
benzoyl-Gly-Ala-Ala-Gly
-
Rattus norvegicus
3.4.24.15
7.9
-
pGlu-Leu-Tyr-Glu-Asn-Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu
-
Rattus norvegicus
3.4.24.15
12
-
pGlu-His-Trp-Ser-Tyr-Gly-Leu-Arg-Pro-Gly-NH2
-
Rattus norvegicus
3.4.24.15
17
-
benzoyl-Gly-Ala-Ala-Leu-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
19.2
-
benzoyl-Gly-Phe-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
33.8
-
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
-
Rattus norvegicus
3.4.24.15
37.9
-
Tyr-Gly-Gly-Phe-Leu-Arg-Lys-Tyr-Pro
-
Rattus norvegicus
3.4.24.15
48.3
-
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
86.4
-
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile
-
Rattus norvegicus
3.4.24.15
94.1
-
tert-butoxycarbonyl-Phe-Ala-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus
3.4.24.15
179
-
benzoyl-Gly-Ala-Ala-Phe-p-aminobenzoate
-
Rattus norvegicus