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Literature summary extracted from

  • Roe, J.L.; Lennarz, W.J.
    Biosynthesis and secretion of the hatching enzyme during sea urchin embryogenesis (1990), J. Biol. Chem., 265, 8704-8711.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.12 1,10-phenanthroline
-
 Strongylocentrotus purpuratus
3.4.24.12 reactive red 120
-
 Strongylocentrotus purpuratus
3.4.24.12 triazine dye
-
 Strongylocentrotus purpuratus
3.4.24.12 Zn2+
-
 Strongylocentrotus purpuratus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.12 Ca2+ required Strongylocentrotus purpuratus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.12 33000
-
 x * 33000, the enzyme is initially secreted as a 57000 Da protein, during purification it is converted to the 33000 Da form, SDS-PAGE Strongylocentrotus purpuratus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.12 egg envelope Strongylocentrotus purpuratus proteolyzes the fertilization envelope, thereby allowing the embryo to hatch ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.12 Strongylocentrotus purpuratus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.12 embryo secretion by the blastula stage embryos during the 2-h period prior to hatching. The biosynthesis of the enzyme begins at the late morula/early blastula stage up to 6 h before secretion Strongylocentrotus purpuratus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.12 additional information
-
-
 Strongylocentrotus purpuratus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.12 egg envelope proteolyzes the fertilization envelope, thereby allowing the embryo to hatch Strongylocentrotus purpuratus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.12 ? x * 33000, the enzyme is initially secreted as a 57000 Da protein, during purification it is converted to the 33000 Da form, SDS-PAGE Strongylocentrotus purpuratus