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Literature summary extracted from

  • Mori, N.; Nikali, T.; Sugihara, H.; Tu, A.T.
    Biochemical characterization of hemorrhagic toxins with fibrinogenase activity isolated from Crotalus ruber ruber venom (1987), Arch. Biochem. Biophys., 253, 108-121.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.1 EDTA
-
 Crotalus ruber ruber
3.4.24.1 o-phenanthroline
-
 Crotalus ruber ruber
3.4.24.42 1,10-phenanthroline
-
 Crotalus ruber ruber
3.4.24.42 EDTA
-
 Crotalus ruber ruber
3.4.24.42 additional information benzamidine, tosyl-L-Phe chloromethyl ketone, soybean or egg white trypsin inhibitor or PCMB Crotalus ruber ruber
3.4.24.48 1,10-phenanthroline
-
 Crotalus ruber ruber
3.4.24.48 EDTA
-
 Crotalus ruber ruber
3.4.24.48 additional information no inhibition by benzamidine, tosyl-L-Phe chloromethyl ketone, soybean or egg white trypsin inhibitor or PCMB Crotalus ruber ruber

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.1 Ca2+ requirement Crotalus ruber ruber
3.4.24.1 K+ requirement Crotalus ruber ruber
3.4.24.1 Mg2+ requirement Crotalus ruber ruber
3.4.24.1 Na+ requirement Crotalus ruber ruber
3.4.24.1 Zn2+ 1 mol of Zn2+ per mol of protein Crotalus ruber ruber
3.4.24.42 Ca2+ 0.75 mol/mol toxin HT-3, 1.1 mol/mol toxin HT-2 Crotalus ruber ruber
3.4.24.42 additional information less than 0.3 mol Mg2+/mol toxin, less than 0.8 mol K+/mol toxin HT-3 and less than 0.9 mol K+/mol toxin HT-2 Crotalus ruber ruber
3.4.24.42 Na+ 8.5 mol/mol toxin HT-3, 10.3 mol/mol toxin HT-2 Crotalus ruber ruber
3.4.24.42 Zn2+ zinc metalloproteinase Crotalus ruber ruber
3.4.24.42 Zn2+ 1 mol zinc/mol enzyme Crotalus ruber ruber
3.4.24.48 Ca2+ metalloproteinase, contains 1.1 mol Ca2+/mol enzyme Crotalus ruber ruber
3.4.24.48 additional information less than 0.3 mol Mg2+/mol enzyme and less than 0.9 mol K+/mol enzyme Crotalus ruber ruber
3.4.24.48 Na+ metalloproteinase, contains 10.3 mol Na+/mol enzyme Crotalus ruber ruber
3.4.24.48 Zn2+ metalloproteinase, contains 1.1 mol Zn2+/mol enzyme Crotalus ruber ruber

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.1 24800
-
 1 * 24800, SDS-PAGE, HT-2 Crotalus ruber ruber
3.4.24.1 25000
-
 HT-2, amino acid analysis Crotalus ruber ruber
3.4.24.1 25500
-
 HT-3, amino acid analysis Crotalus ruber ruber
3.4.24.1 25700
-
 1 * 25700, SDS-PAGE, HT-3 Crotalus ruber ruber
3.4.24.1 60000
-
 1 * 60000, SDS-PAGE, HT-1 Crotalus ruber ruber
3.4.24.1 60050
-
 Ht-1, amino acid analysis Crotalus ruber ruber
3.4.24.42 additional information
-
 amino acid composition Crotalus ruber ruber
3.4.24.42 25000
-
 1 * 25000, Crotalus ruber ruber, toxin HT-2 Crotalus ruber ruber
3.4.24.42 25500
-
 1 * 25500, Crotalus ruber ruber, toxin HT-3 Crotalus ruber ruber
3.4.24.48 additional information
-
 amino acid composition of isozyme HT-2 Crotalus ruber ruber
3.4.24.48 25000
-
 x * 25000, Crotalus ruber ruber, isozyme HT-2, SDS-PAGE Crotalus ruber ruber

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.1 Proteins + H2O Crotalus ruber ruber preferential cleavage: -Leu, -Ile, -Val, -Phe ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.1 Crotalus ruber ruber
-
 red rattlesnake
-
3.4.24.42 Crotalus ruber ruber
-
 red rattlesnake
-
3.4.24.48 Crotalus ruber ruber
-
 red rattlesnake
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.1 HT-1, HT-2 and HT-3 Crotalus ruber ruber
3.4.24.42 from lyophilized crude venom, toxins HT-2 and HT-3 Crotalus ruber ruber
3.4.24.48 from lyophilized crude venom Crotalus ruber ruber

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.1 venom
-
 Crotalus ruber ruber
-
3.4.24.42 venom
-
 Crotalus ruber ruber
-
3.4.24.48 venom
-
 Crotalus ruber ruber
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.1 alpha fibrinogen + H2O
-
 Crotalus ruber ruber ?
-
 ?
3.4.24.1 angiotensin I + H2O
-
 Crotalus ruber ruber ?
-
 ?
3.4.24.1 beta fibrinogen + H2O HT-1 Crotalus ruber ruber ?
-
 ?
3.4.24.1 demethylcasein + H2O
-
 Crotalus ruber ruber ?
-
 ?
3.4.24.1 insulin B chain + H2O
-
 Crotalus ruber ruber ?
-
 ?
3.4.24.1 Proteins + H2O preferential cleavage: -Leu, -Ile, -Val, -Phe Crotalus ruber ruber ?
-
 ?
3.4.24.42 angiotensin I + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, cleaved at His-Pro and Pro-Phe Crotalus ruber ruber Asp-Arg-Val-Tyr-Ile-His + Pro + Phe-His-Leu
-
 ?
3.4.24.42 casein + H2O
-
 Crotalus ruber ruber ?
-
 ?
3.4.24.42 Dimethylcasein + H2O
-
 Crotalus ruber ruber ?
-
 ?
3.4.24.42 hide powder azure + H2O
-
 Crotalus ruber ruber ?
-
 ?
3.4.24.42 Luteinizing hormone-releasing hormone + H2O cleavage sites: Trp3-Ser4, Gly6-Leu7 Crotalus ruber ruber ?
-
 ?
3.4.24.42 Met-enkephalin + H2O i.e. Tyr-Gly-Gly-Phe-Met, cleavage site: Gly3-Phe4 Crotalus ruber ruber Tyr-Gly-Gly + Phe-Met
-
 ?
3.4.24.42 additional information hemorrhagic and myonecrotic activity Crotalus ruber ruber ?
-
 ?
3.4.24.42 Oxidized insulin B-chain + H2O cleavage at Gly23-Phe24 Crotalus ruber ruber ?
-
 ?
3.4.24.42 Oxidized insulin B-chain + H2O Crotalus ruber ruber toxin HT-3 does not cleave the His5-Leu6 bond which is cleaved by HT-2 Crotalus ruber ruber ?
-
 ?
3.4.24.48 angiotensin I + H2O i.e. Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu, cleaved at His-Pro and Pro-Phe Crotalus ruber ruber ?
-
 ?
3.4.24.48 casein + H2O
-
 Crotalus ruber ruber ?
-
 ?
3.4.24.48 Dimethylcasein + H2O
-
 Crotalus ruber ruber ?
-
 ?
3.4.24.48 Fibrin + H2O
-
 Crotalus ruber ruber ?
-
 ?
3.4.24.48 Fibrinogen + H2O Aalpha chain, not beta or gamma chain Crotalus ruber ruber ?
-
 ?
3.4.24.48 hide powder azure + H2O
-
 Crotalus ruber ruber ?
-
 ?
3.4.24.48 Luteinizing hormone-releasing hormone + H2O cleavage sites: Trp3-Ser4, Gly6-Leu7 Crotalus ruber ruber ?
-
 ?
3.4.24.48 Met-enkephalin + H2O i.e. Tyr-Gly-Gly-Phe-Met, cleavage site: Gly3-Phe4 Crotalus ruber ruber Tyr-Gly-Gly + Phe-Met
-
 ?
3.4.24.48 additional information exhibits proteolytic, hemorrhatic and myonecrotic activity Crotalus ruber ruber ?
-
 ?
3.4.24.48 Oxidized insulin B-chain + H2O cleavage sites: His10-Leu11, Ala14-Leu15, Tyr16-Leu17, Gly23-Phe24 Crotalus ruber ruber Hydrolyzed insulin B-chain
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.1 monomer 1 * 24800, SDS-PAGE, HT-2 Crotalus ruber ruber
3.4.24.1 monomer 1 * 60000, SDS-PAGE, HT-1 Crotalus ruber ruber
3.4.24.1 monomer 1 * 25700, SDS-PAGE, HT-3 Crotalus ruber ruber
3.4.24.42 monomer 1 * 25000, Crotalus ruber ruber, toxin HT-2 Crotalus ruber ruber
3.4.24.42 monomer 1 * 25500, Crotalus ruber ruber, toxin HT-3 Crotalus ruber ruber
3.4.24.48 ? x * 25000, Crotalus ruber ruber, isozyme HT-2, SDS-PAGE Crotalus ruber ruber

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.42 70
-
 inactivation Crotalus ruber ruber

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.42 additional information
-
 pI: 5.2 (toxin HT-2), pI: 9.6 (toxin HT-3) Crotalus ruber ruber
3.4.24.48 additional information
-
 pI: 5.2 Crotalus ruber ruber