BRENDA - Enzyme Database show

Purification and properties of cathepsin D from porcine spleen

Cunningham, M.; Tang, J.; J. Biol. Chem. 251, 4528-4536 (1976)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
3.4.23.5
stable upon freezing
Sus scrofa
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.23.5
1,2-epoxy-3-(4-nitrophenoxy)propane
-
Sus scrofa
3.4.23.5
Diazoacetyl-DL-norleucine methyl ester
-
Sus scrofa
3.4.23.5
ethanol
5%
Sus scrofa
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.4.23.5
Sus scrofa
-
pig
-
Purification (Commentary)
EC Number
Commentary
Organism
3.4.23.5
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.23.5
spleen
-
Sus scrofa
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.23.5
Hemoglobin + H2O
-
36924
Sus scrofa
?
-
-
-
?
3.4.23.5
insulin + H2O
oxidized A chain and B chain
36924
Sus scrofa
?
-
-
-
?
3.4.23.5
peptide D + H2O
-
36924
Sus scrofa
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.4.23.5
?
x * 34000-35000, SDS-PAGE
Sus scrofa
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.4.23.5
50
-
stable up to
Sus scrofa
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.23.5
2.9
-
0.1 M sodium acetate or formate buffer, hydrolysis of hemoglobin
Sus scrofa
3.4.23.5
3.4
-
2 pH-optima: at pH 3.4 and at pH 3.8, 0.5 M sodium acetate or formate buffer, hydrolysis of hemoglobin
Sus scrofa
3.4.23.5
3.8
-
2 pH-optima: at pH 3.4 and at pH 3.8, 0.5 M sodium acetate or formate buffer, hydrolysis of hemoglobin
Sus scrofa
General Stability (protein specific)
EC Number
General Stability
Organism
3.4.23.5
stable upon freezing
Sus scrofa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.23.5
1,2-epoxy-3-(4-nitrophenoxy)propane
-
Sus scrofa
3.4.23.5
Diazoacetyl-DL-norleucine methyl ester
-
Sus scrofa
3.4.23.5
ethanol
5%
Sus scrofa
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.23.5
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.23.5
spleen
-
Sus scrofa
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.23.5
Hemoglobin + H2O
-
36924
Sus scrofa
?
-
-
-
?
3.4.23.5
insulin + H2O
oxidized A chain and B chain
36924
Sus scrofa
?
-
-
-
?
3.4.23.5
peptide D + H2O
-
36924
Sus scrofa
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.23.5
?
x * 34000-35000, SDS-PAGE
Sus scrofa
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
3.4.23.5
50
-
stable up to
Sus scrofa
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.23.5
2.9
-
0.1 M sodium acetate or formate buffer, hydrolysis of hemoglobin
Sus scrofa
3.4.23.5
3.4
-
2 pH-optima: at pH 3.4 and at pH 3.8, 0.5 M sodium acetate or formate buffer, hydrolysis of hemoglobin
Sus scrofa
3.4.23.5
3.8
-
2 pH-optima: at pH 3.4 and at pH 3.8, 0.5 M sodium acetate or formate buffer, hydrolysis of hemoglobin
Sus scrofa