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Literature summary extracted from

  • Krieger, T.J.; Hook, V.Y.H.
    Purification and characterization of a cathepsin D protease from bovine chromaffin granule (1992), Biochemistry, 31, 4223-4231.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.23.5 stable against repetitive freezing and thawing Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.5 pepstatin pepstatin A Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.23.5 chromaffin granule
-
 Bos taurus 42583
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.5 45000 55000 two enzyme forms exist: a single 47000 Da polypeptide and a complex of 30000 Da subunit plus a 16500 Da subunit, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.5 additional information Bos taurus possible participation of the enzyme in cleaving some peptide precursors ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.5 Bos taurus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.23.5 side-chain modification glycoprotein Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.5
-
 Bos taurus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.23.5 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.4.23.5 -70°C, stable Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.5 additional information cleaves at paired basic residues and at hydrophobic residues Bos taurus ?
-
 ?
3.4.23.5 additional information possible participation of the enzyme in cleaving some peptide precursors Bos taurus ?
-
 ?
3.4.23.5 neuropeptide K + H2O cleaved at a Lys-Arg basic residue site Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.5 ? two enzyme forms exist: a single 47000 Da polypeptide and a complex of 30000 Da subunit plus a 16500 Da subunit, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.5 5 5.5
-
 Bos taurus