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Literature summary extracted from

  • Kalousek, F.; Konigsberg, W.H.
    Purification and characterization of histidyl transfer ribonucleic acid synthetase of Escherichia coli (1974), Biochemistry, 13, 999-1006.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.1.1.21 2-mercaptoethanol stabilizes the enzyme Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.21 1,2,4-Triazole-3-alanine
-
 Escherichia coli
6.1.1.21 D-histidine
-
 Escherichia coli
6.1.1.21 diphosphate weak Escherichia coli
6.1.1.21 L-histidinol
-
 Escherichia coli
6.1.1.21 Mg2+ inhibition at 15 mM, optimal activation at 5-10 mM Escherichia coli
6.1.1.21 N-Acetylhistidine
-
 Escherichia coli
6.1.1.21 NEM
-
 Escherichia coli
6.1.1.21 p-chloromercuribenzoate L-His, ATP, and Mg2+ protect Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.21 Mg2+ optimal concentration: 5-10 mM, inhibition at 15 mM Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.21 42500
-
 2 * 42500, SDS-PAGE Escherichia coli
6.1.1.21 85000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.21 Escherichia coli
-
 K12
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.21 ATP + L-histidine + tRNAHis = AMP + diphosphate + L-histidyl-tRNAHis two molecules of ATP and histidine are bound per molecule of enzyme Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.21 121.8
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
6.1.1.21 -20°C, 50% glycerol, 5 mM 2-mercaproethanol, stable for more than 4 months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.21 ATP + L-histidine + tRNAHis
-
 Escherichia coli AMP + diphosphate + L-histidyl-tRNAHis
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.21 dimer 2 * 42500, SDS-PAGE Escherichia coli
6.1.1.21 More at temperatures above 25 C, the enzyme tends to aggregate giving forms with a MW 90000 and 175000 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.21 37
-
 in presence of 2-mercaptoethanol stable for 5 h, 40% loss of activity after 12 h. In absence of 2-mercaptoethanol total loss of activity in a few min Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.21 7.4
-
 histidyl-tRNA formation Escherichia coli
6.1.1.21 8 9 histidine-dependent ATP-diphosphate exchange Escherichia coli