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Literature summary extracted from
- Protein engineering of chymosin and expression in Trichoderma reesei (1991), Food Biotechnol., 19, 663-666.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.23.4 | point mutants expressed in Escherichia coli and Trichoderma reesei | Bos taurus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.4.23.4 | D304A/T218A | double mutant, substrate-specificity-pocket variant | Bos taurus |
| 3.4.23.4 | Q288K | - |
Bos taurus |
| 3.4.23.4 | T218A | pH optima shift | Bos taurus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.4 | kappa-casein + H2O | Bos taurus | cleaves a single bond in kappa-casein between phenylalanine 105 and methionine 106 | ? | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.4 | Bos taurus | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.4 | kappa-casein + H2O | cleaves a single bond in kappa-casein between phenylalanine 105 and methionine 106 | Bos taurus | ? | - |
? | |
| 3.4.23.4 | Lys-Pro-Ala-Glu-Phe-Phe(NO2)-Ala-Leu-OH + H2O | - |
Bos taurus | Lys-Pro-Ala-Glu-Phe + Phe(NO2)-Ala-Leu | - |
? | |
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Release 2023.1 (January 2023)
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