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Literature summary extracted from

  • Miyoshi, M.; Yoon, C.H.; Ibuki, F.; Kanamori, M.
    The characterization of rennin action on kappa-casein using CM-cellulose (1976), Agric. Biol. Chem., 40, 347-352.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.4 0.89
-
 kappa-casein
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.23.4 kappa-casein + H2O Bos taurus cleaves a single bond in kappa-casein between phenylalanine 105 and methionine 106 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.4 Bos taurus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.4 kappa-casein + H2O cleaves a single bond in kappa-casein between phenylalanine 105 and methionine 106 Bos taurus p-kappa-casein + glycomacropeptide p-kappa-casein i.e. N-terminal fraction, glycomacropeptide i.e. C-terminal fraction ?
3.4.23.4 kappa-casein + H2O cleaves a single bond in kappa-casein between phenylalanine 105 and methionine 106 Bos taurus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.4 50
-
-
 Bos taurus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.23.4 20 60 20°C: about 25% of maximum activity, 60°C: about 50% of maximum activity Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.4 5
-
-
 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.23.4 3 10 pH 3: about 80% of maximum activity, pH 10: about 20% of maximum activity Bos taurus