BRENDA - Enzyme Database

Catalytic properties of the cysteine aminopeptidase PepC, a bacterial bleomycin hydrolase

Mistou, M.Y.; Gripon, J.C.; Biochim. Biophys. Acta 1383, 63-70 (1998) View publication on PubMed

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.22.40
0.13
-
Gly-Gly-Gly-Ala
-
Lactococcus lactis
3.4.22.40
0.2
-
Gly-Gly-Phe-Leu
-
Lactococcus lactis
3.4.22.40
0.29
-
Gly-Gly-Phe
-
Lactococcus lactis
3.4.22.40
0.46
-
bleomycin A2
-
Lactococcus lactis
3.4.22.40
0.5
-
Met-Ala-Ser
-
Lactococcus lactis
3.4.22.40
0.57
-
Gly-Gly-Leu
-
Lactococcus lactis
3.4.22.40
0.63
-
Thr-Ser-Lys
-
Lactococcus lactis
3.4.22.40
0.66
-
Gly-Gly-Ala
-
Lactococcus lactis
3.4.22.40
0.75
-
Gly-Gly-Arg
-
Lactococcus lactis
3.4.22.40
0.78
-
Gly-Gly-Gly-Gly
-
Lactococcus lactis
3.4.22.40
1.01
-
Gly-Gly-D-Leu
-
Lactococcus lactis
3.4.22.40
1.05
-
Gly-Gly-Gly
-
Lactococcus lactis
3.4.22.40
1.05
-
Gly-Gly-Tyr
-
Lactococcus lactis
3.4.22.40
1.14
-
Gly-Gly-Glu
-
Lactococcus lactis
3.4.22.40
1.24
-
Gly-Gly-Gly-Gly
-
Lactococcus lactis
3.4.22.40
1.7
-
Gly-Phe
-
Lactococcus lactis
3.4.22.40
2.7
-
Thr-Ser
-
Lactococcus lactis
3.4.22.40
2.9
-
Gly-Ala
-
Lactococcus lactis
3.4.22.40
3.5
-
Phe-p-nitroanilide
-
Lactococcus lactis
3.4.22.40
4.1
-
Gly-Gly-NH2
-
Lactococcus lactis
3.4.22.40
4.6
-
Gly-Leu
-
Lactococcus lactis
3.4.22.40
4.7
-
Gly-Lys
-
Lactococcus lactis
3.4.22.40
5.6
-
Gly-D-Leu
-
Lactococcus lactis
3.4.22.40
6.1
-
Arg-p-nitroanilide
-
Lactococcus lactis
3.4.22.40
7
-
Met-paranitroanilide
-
Lactococcus lactis
3.4.22.40
9.5
-
Met-Ala
-
Lactococcus lactis
3.4.22.40
9.7
-
Gly-p-nitroanilide
-
Lactococcus lactis
3.4.22.40
9.9
-
Lys-p-nitroanilide
-
Lactococcus lactis
3.4.22.40
10.1
-
Gly-Gly
-
Lactococcus lactis
3.4.22.40
12.5
-
Leu-p-nitroanilide
-
Lactococcus lactis
3.4.22.40
13.5
-
Gly-NH2
-
Lactococcus lactis
3.4.22.40
14.8
-
Gly-Asp
-
Lactococcus lactis
3.4.22.40
17.4
-
Ala-p-nitroanilide
-
Lactococcus lactis
3.4.22.40
28.4
-
Asp-p-nitroanilide
-
Lactococcus lactis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.22.40
Lactococcus lactis
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.22.40
Ala-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Ala + 4-nitroaniline
-
-
-
?
3.4.22.40
Arg-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Arg + 4-nitroaniline
-
-
-
?
3.4.22.40
Asp-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Asp + 4-nitroaniline
-
-
-
?
3.4.22.40
bleomycin + H2O
bleomycin A2
36712
Lactococcus lactis
deamido-bleomycin
-
36712
Lactococcus lactis
?
3.4.22.40
bleomycin A2 + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Ala + H2O
-
36712
Lactococcus lactis
Gly + Ala
-
-
-
?
3.4.22.40
Gly-Asp + H2O
-
36712
Lactococcus lactis
Gly + Asp
-
-
-
?
3.4.22.40
Gly-D-Leu + H2O
-
36712
Lactococcus lactis
Gly + D-Leu
-
-
-
?
3.4.22.40
Gly-Gly + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Ala + H2O
-
36712
Lactococcus lactis
glycyl-L-alanine + glycine
-
-
-
?
3.4.22.40
Gly-Gly-Arg + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-D-Leu + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Glu + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Gly + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Gly-Ala + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Gly-Gly + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Gly-Gly-Gly + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Leu + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-NH2 + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Phe + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Phe-Leu + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Tyr + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Leu + H2O
-
36712
Lactococcus lactis
Gly + Leu
-
-
-
?
3.4.22.40
Gly-Lys + H2O
-
36712
Lactococcus lactis
Gly + Lys
-
-
-
?
3.4.22.40
Gly-NH2 + H2O
-
36712
Lactococcus lactis
Gly + NH3
-
-
-
?
3.4.22.40
Gly-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Gly + 4-nitroaniline
-
-
-
?
3.4.22.40
Gly-Phe + H2O
-
36712
Lactococcus lactis
Gly + Phe
-
-
-
?
3.4.22.40
Leu-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Leu + 4-nitroaniline
-
-
-
?
3.4.22.40
Lys-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Lys + 4-nitroaniline
-
-
-
?
3.4.22.40
Met-Ala + H2O
-
36712
Lactococcus lactis
Met + Ala
-
-
-
?
3.4.22.40
Met-Ala-Ser + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Met-p-itroanilide + H2O
-
36712
Lactococcus lactis
Met + 4-nitroaniline
-
-
-
?
3.4.22.40
Phe-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Phe + 4-nitroaniline
-
-
-
?
3.4.22.40
Thr-Ser + H2O
-
36712
Lactococcus lactis
Thr + Ser
-
-
-
?
3.4.22.40
Thr-Ser-Lys + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.22.40
7
-
-
Lactococcus lactis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.22.40
6
8
-
Lactococcus lactis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.22.40
0.13
-
Gly-Gly-Gly-Ala
-
Lactococcus lactis
3.4.22.40
0.2
-
Gly-Gly-Phe-Leu
-
Lactococcus lactis
3.4.22.40
0.29
-
Gly-Gly-Phe
-
Lactococcus lactis
3.4.22.40
0.46
-
bleomycin A2
-
Lactococcus lactis
3.4.22.40
0.5
-
Met-Ala-Ser
-
Lactococcus lactis
3.4.22.40
0.57
-
Gly-Gly-Leu
-
Lactococcus lactis
3.4.22.40
0.63
-
Thr-Ser-Lys
-
Lactococcus lactis
3.4.22.40
0.66
-
Gly-Gly-Ala
-
Lactococcus lactis
3.4.22.40
0.75
-
Gly-Gly-Arg
-
Lactococcus lactis
3.4.22.40
0.78
-
Gly-Gly-Gly-Gly
-
Lactococcus lactis
3.4.22.40
1.01
-
Gly-Gly-D-Leu
-
Lactococcus lactis
3.4.22.40
1.05
-
Gly-Gly-Gly
-
Lactococcus lactis
3.4.22.40
1.05
-
Gly-Gly-Tyr
-
Lactococcus lactis
3.4.22.40
1.14
-
Gly-Gly-Glu
-
Lactococcus lactis
3.4.22.40
1.24
-
Gly-Gly-Gly-Gly
-
Lactococcus lactis
3.4.22.40
1.7
-
Gly-Phe
-
Lactococcus lactis
3.4.22.40
2.7
-
Thr-Ser
-
Lactococcus lactis
3.4.22.40
2.9
-
Gly-Ala
-
Lactococcus lactis
3.4.22.40
3.5
-
Phe-p-nitroanilide
-
Lactococcus lactis
3.4.22.40
4.1
-
Gly-Gly-NH2
-
Lactococcus lactis
3.4.22.40
4.6
-
Gly-Leu
-
Lactococcus lactis
3.4.22.40
4.7
-
Gly-Lys
-
Lactococcus lactis
3.4.22.40
5.6
-
Gly-D-Leu
-
Lactococcus lactis
3.4.22.40
6.1
-
Arg-p-nitroanilide
-
Lactococcus lactis
3.4.22.40
7
-
Met-paranitroanilide
-
Lactococcus lactis
3.4.22.40
9.5
-
Met-Ala
-
Lactococcus lactis
3.4.22.40
9.7
-
Gly-p-nitroanilide
-
Lactococcus lactis
3.4.22.40
9.9
-
Lys-p-nitroanilide
-
Lactococcus lactis
3.4.22.40
10.1
-
Gly-Gly
-
Lactococcus lactis
3.4.22.40
12.5
-
Leu-p-nitroanilide
-
Lactococcus lactis
3.4.22.40
13.5
-
Gly-NH2
-
Lactococcus lactis
3.4.22.40
14.8
-
Gly-Asp
-
Lactococcus lactis
3.4.22.40
17.4
-
Ala-p-nitroanilide
-
Lactococcus lactis
3.4.22.40
28.4
-
Asp-p-nitroanilide
-
Lactococcus lactis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.22.40
Ala-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Ala + 4-nitroaniline
-
-
-
?
3.4.22.40
Arg-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Arg + 4-nitroaniline
-
-
-
?
3.4.22.40
Asp-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Asp + 4-nitroaniline
-
-
-
?
3.4.22.40
bleomycin + H2O
bleomycin A2
36712
Lactococcus lactis
deamido-bleomycin
-
36712
Lactococcus lactis
?
3.4.22.40
bleomycin A2 + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Ala + H2O
-
36712
Lactococcus lactis
Gly + Ala
-
-
-
?
3.4.22.40
Gly-Asp + H2O
-
36712
Lactococcus lactis
Gly + Asp
-
-
-
?
3.4.22.40
Gly-D-Leu + H2O
-
36712
Lactococcus lactis
Gly + D-Leu
-
-
-
?
3.4.22.40
Gly-Gly + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Ala + H2O
-
36712
Lactococcus lactis
glycyl-L-alanine + glycine
-
-
-
?
3.4.22.40
Gly-Gly-Arg + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-D-Leu + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Glu + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Gly + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Gly-Ala + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Gly-Gly + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Gly-Gly-Gly + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Leu + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-NH2 + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Phe + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Phe-Leu + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Gly-Tyr + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Gly-Leu + H2O
-
36712
Lactococcus lactis
Gly + Leu
-
-
-
?
3.4.22.40
Gly-Lys + H2O
-
36712
Lactococcus lactis
Gly + Lys
-
-
-
?
3.4.22.40
Gly-NH2 + H2O
-
36712
Lactococcus lactis
Gly + NH3
-
-
-
?
3.4.22.40
Gly-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Gly + 4-nitroaniline
-
-
-
?
3.4.22.40
Gly-Phe + H2O
-
36712
Lactococcus lactis
Gly + Phe
-
-
-
?
3.4.22.40
Leu-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Leu + 4-nitroaniline
-
-
-
?
3.4.22.40
Lys-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Lys + 4-nitroaniline
-
-
-
?
3.4.22.40
Met-Ala + H2O
-
36712
Lactococcus lactis
Met + Ala
-
-
-
?
3.4.22.40
Met-Ala-Ser + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
3.4.22.40
Met-p-itroanilide + H2O
-
36712
Lactococcus lactis
Met + 4-nitroaniline
-
-
-
?
3.4.22.40
Phe-p-nitroanilide + H2O
-
36712
Lactococcus lactis
Phe + 4-nitroaniline
-
-
-
?
3.4.22.40
Thr-Ser + H2O
-
36712
Lactococcus lactis
Thr + Ser
-
-
-
?
3.4.22.40
Thr-Ser-Lys + H2O
-
36712
Lactococcus lactis
?
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.22.40
7
-
-
Lactococcus lactis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.22.40
6
8
-
Lactococcus lactis