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Literature summary extracted from

  • Barrett, A.J.; Kirschke, H.
    Cathepsin B, Cathepsin H, and cathepsin L (1981), Methods Enzymol., 80, 535-561.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.16 alpha-2-Macroglobulin
-
 Homo sapiens
3.4.22.16 ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate E-64-d, inhibits processing of proenzyme to synthetic analogs Homo sapiens
3.4.22.16 ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate
-
 Rattus norvegicus
3.4.22.16 leupeptin low sensitivity Homo sapiens
3.4.22.16 leupeptin Ki: 0.005 mM; low sensitivity Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.16 0.097
-
 Arg-2-naphthylamide
-
 Homo sapiens
3.4.22.16 0.15
-
 Arg-4-methylcoumaryl-7-amide
-
 Homo sapiens
3.4.22.16 0.637
-
 benzoyl-Arg-2-naphthylamide
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.1 lysosome
-
 Homo sapiens 5764
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.16 28000
-
 gel filtration Homo sapiens
3.4.22.16 28000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.1 Proteins + H2O Homo sapiens
-
 ?
-
 ?
3.4.22.16 Proteins + H2O Homo sapiens aminopeptidase and endopeptidase activity ?
-
 ?
3.4.22.16 Proteins + H2O Rattus norvegicus aminopeptidase and endopeptidase activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Homo sapiens
-
-
-
3.4.22.16 Homo sapiens
-
-
-
3.4.22.16 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.16 side-chain modification
-
 Homo sapiens
3.4.22.16 side-chain modification
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.1
-
 Homo sapiens
3.4.22.16 2 methods Homo sapiens
3.4.22.16 two methods Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 liver
-
 Homo sapiens
-
3.4.22.16 liver
-
 Homo sapiens
-
3.4.22.16 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.4.22.16 4°C or -20°C, 50 mM Na-acetate buffer, pH 5.0, 0.2 M NaCl, 0. 5 mM HgCl2, 1 mM EDTA Homo sapiens
3.4.22.16 4°C or -20°C, 50 mM Na-acetate buffer, pH 5.0, 0.2 M NaCl, 0. 5 mM HgCl2, 1 mM EDTA Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O
-
 Homo sapiens benzoyl-DL-Arg + beta-naphthylamine
-
 ?
3.4.22.1 benzoyl-DL-Arg-p-nitroanilide + H2O
-
 Homo sapiens benzoyl-DL-Arg + p-nitroaniline
-
 ?
3.4.22.1 benzyloxycarbonyl-Arg-Arg-4-methylcoumarin 7-amide + H2O
-
 Homo sapiens benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 benzyloxycarbonyl-Arg-Arg-beta-naphthylamide + H2O
-
 Homo sapiens benzyloxycarbonyl-Arg-Arg + beta-naphthylamine
-
 ?
3.4.22.1 benzyloxycarbonyl-Phe-Arg-4-methylcoumarin-7-amide
-
 Homo sapiens benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.22.1 Proteins + H2O
-
 Homo sapiens ?
-
 ?
3.4.22.16 alpha-N-benzoyl-DL-arginine-beta-naphthylamide + H2O
-
 Homo sapiens alpha-N-benzoyl-DL-arginine + beta-naphthylamine
-
 ?
3.4.22.16 Arg-2-naphthylamide + H2O
-
 Homo sapiens Arg + 2-naphthylamine
-
 ir
3.4.22.16 Arg-2-naphthylamide + H2O
-
 Rattus norvegicus Arg + 2-naphthylamine
-
 ir
3.4.22.16 Arg-4-methylcoumaryl-7-amide + H2O conventional test substrate Homo sapiens Arg + 7-amino-4-methylcoumarin
-
 ir
3.4.22.16 Arg-4-methylcoumaryl-7-amide + H2O conventional test substrate Rattus norvegicus Arg + 7-amino-4-methylcoumarin
-
 ir
3.4.22.16 benzoyl-DL-Arg-2-naphthylamide + H2O
-
 Homo sapiens benzoyl-DL-Arg + 2-naphthylamine
-
 ir
3.4.22.16 benzoyl-DL-Arg-2-naphthylamide + H2O
-
 Rattus norvegicus benzoyl-DL-Arg + 2-naphthylamine
-
 ir
3.4.22.16 Leu-2-naphthylamide + H2O
-
 Homo sapiens Leu + 2-naphthylamine
-
 ir
3.4.22.16 Leu-2-naphthylamide + H2O
-
 Rattus norvegicus Leu + 2-naphthylamine
-
 ir
3.4.22.16 additional information activity as endopeptidase and aminopeptidase on synthetic substrates Homo sapiens ?
-
 ?
3.4.22.16 additional information activity as endopeptidase and aminopeptidase on synthetic substrates Rattus norvegicus ?
-
 ?
3.4.22.16 Proteins + H2O aminopeptidase and endopeptidase activity Homo sapiens ?
-
 ?
3.4.22.16 Proteins + H2O aminopeptidase and endopeptidase activity Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.1 dimer heavy and light chain result from cleavage of mature cathepsin B Homo sapiens
3.4.22.16 monomer
-
 Homo sapiens
3.4.22.16 monomer
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.16 40
-
 assay at Homo sapiens
3.4.22.16 40
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.16 1.82
-
 Arg-2-naphthylamide
-
 Homo sapiens
3.4.22.16 1.82
-
 benzoyl-Arg-2-naphthylamide
-
 Homo sapiens
3.4.22.16 2.53
-
 Arg-4-methylcoumaryl-7-amide
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.1 6
-
-
 Homo sapiens
3.4.22.16 5 6 substrate azocasein Homo sapiens
3.4.22.16 5 6 substrate azocasein Rattus norvegicus
3.4.22.16 6.8
-
-
 Rattus norvegicus
3.4.22.16 6.8
-
 synthetic substrates Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.16 7
-
 irreversible inactivation above Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.16 0.005
-
 leupeptin
-
 Rattus norvegicus