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Literature summary extracted from

  • Takahashi, T.; Yonezawa, S.; Dehdarani, A.H.; Tang, J.
    Comparative studies of two cathepsin B isozymes from porcine spleen. Isolation, polypeptide chain arrangements, and enzyme specificity (1986), J. Biol. Chem., 261, 9368-9374.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.1 0.066
-
 benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-beta-naphthylamide isoenzyme II Sus scrofa
3.4.22.1 0.07
-
 benzoyl-Phe-Val-Arg-p-nitroanilide isoenzyme I Sus scrofa
3.4.22.1 0.072
-
 N-t-butyloxycarbonyl-Gln-p-nitrophenyl ester isoenzyme I and isoenzyme II Sus scrofa
3.4.22.1 0.13
-
 benzoyl-Phe-Val-Arg-p-nitroanilide isoenzyme II Sus scrofa
3.4.22.1 0.13
-
 benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-beta-naphthylamide isoenzyme I Sus scrofa
3.4.22.1 0.2
-
 benzyloxycarbonyl-Val-Lys-Lys-Arg-4-methoxy-beta-naphthylamide isoenzyme I Sus scrofa
3.4.22.1 0.43
-
 benzoyl-Pro-Phe-Arg-p-nitroanilide isoenzyme I Sus scrofa
3.4.22.1 0.8
-
 benzoyl-DL-Arg-beta-naphthylamide isoenzyme II Sus scrofa
3.4.22.1 0.91
-
 benzyloxycarbonyl-Val-Lys-Lys-Arg-4-methoxy-beta-naphthylamide isoenzyme II Sus scrofa
3.4.22.1 1
-
 benzoyl-Pro-Phe-Arg-p-nitroanilide isoenzyme II Sus scrofa
3.4.22.1 2.9
-
 benzoyl-DL-Arg-beta-naphthylamide isoenzyme I Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.1 23000
-
 isoenzyme I, 1 * 5000 + 1 * 23000, gel filtration, SDS-PAGE Sus scrofa
3.4.22.1 27000
-
 isoenzyme II, 1 * 27000, gel filtration, SDS-PAGE Sus scrofa
3.4.22.1 27000 33000 isoenzyme II, gel filtration, SDS-PAGE Sus scrofa
3.4.22.1 28000
-
 isoenzyme I Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.1 Proteins + H2O Sus scrofa degradation of tissue proteins within lysosomes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.1 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.1 side-chain modification glycoprotein Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.1 spleen
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.1 benzoyl-DL-Arg-beta-naphthylamide + H2O
-
 Sus scrofa benzoyl-DL-Arg + beta-naphthylamine
-
 ?
3.4.22.1 benzoyl-Phe-Val-Arg-p-nitroanilide + H2O
-
 Sus scrofa benzoyl-Phe-Val-Arg + p-nitroaniline
-
 ?
3.4.22.1 benzoyl-Pro-Phe-Arg-p-nitroanilide + H2O
-
 Sus scrofa benzoyl-Pro-Phe-Arg + p-nitroaniline
-
 ?
3.4.22.1 benzyloxycarbonyl-Ala-Arg-Arg-4-methoxy-beta-naphthylamide + H2O
-
 Sus scrofa benzyloxycarbonyl-Ala-Arg-Arg + 4-methoxy-beta-naphthylamine
-
 ?
3.4.22.1 benzyloxycarbonyl-Val-Lys-Lys-Arg-4-methoxy-beta-naphthylamide + H2O
-
 Sus scrofa ? + 4-methoxy-beta-naphthylamine
-
 ?
3.4.22.1 Glucagon + H2O
-
 Sus scrofa Hydrolyzed glucagon
-
 ?
3.4.22.1 N-t-butyloxycarbonyl-Gln-p-nitrophenyl ester + H2O
-
 Sus scrofa N-t-butyloxycarbonyl-Gln + 4-nitrophenol
-
 ?
3.4.22.1 Proteins + H2O degradation of tissue proteins within lysosomes Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.1 dimer isoenzyme I, 1 * 5000 + 1 * 23000, gel filtration, SDS-PAGE Sus scrofa
3.4.22.1 monomer isoenzyme II, 1 * 27000, gel filtration, SDS-PAGE Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.1 6.1
-
-
 Sus scrofa