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Literature summary extracted from

  • Sakiyama, F.; Masaki, T.
    Lysyl endopeptidase of Achromobacter lyticus (1994), Methods Enzymol., 244, 126-137.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.50 the cloned gene of prepro-API is expressed in Escherichia coli, pro-API is secreted into the periplasm and activated autocatalytically Achromobacter lyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.50 6-amino-1-hexanoic acid
-
 Achromobacter lyticus
3.4.21.50 acetonitrile
-
 Achromobacter lyticus
3.4.21.50 Ba2+
-
 Achromobacter lyticus
3.4.21.50 benzoyl-DL-lysinal
-
 Achromobacter lyticus
3.4.21.50 Benzoyl-Lys
-
 Achromobacter lyticus
3.4.21.50 benzyloxycarbonyl-Leu-Leu-lysinal
-
 Achromobacter lyticus
3.4.21.50 benzyloxycarbonyl-Pro-lysinal
-
 Achromobacter lyticus
3.4.21.50 benzyloxycarbonyl-Val-lysinal
-
 Achromobacter lyticus
3.4.21.50 Cs+
-
 Achromobacter lyticus
3.4.21.50 Cyclohexylamine
-
 Achromobacter lyticus
3.4.21.50 diisopropylfluorophosphate
-
 Achromobacter lyticus
3.4.21.50 ethylamine
-
 Achromobacter lyticus
3.4.21.50 hydrazine
-
 Achromobacter lyticus
3.4.21.50 Isobutylamine
-
 Achromobacter lyticus
3.4.21.50 K+
-
 Achromobacter lyticus
3.4.21.50 Li+
-
 Achromobacter lyticus
3.4.21.50 Lys
-
 Achromobacter lyticus
3.4.21.50 methylamine
-
 Achromobacter lyticus
3.4.21.50 n-alkylamine
-
 Achromobacter lyticus
3.4.21.50 n-amylamine
-
 Achromobacter lyticus
3.4.21.50 n-butylamine
-
 Achromobacter lyticus
3.4.21.50 n-Hexylamine
-
 Achromobacter lyticus
3.4.21.50 n-Propylamine
-
 Achromobacter lyticus
3.4.21.50 Na+
-
 Achromobacter lyticus
3.4.21.50 Nalpha-p-tosyl-L-Lys-chloromethylketone
-
 Achromobacter lyticus
3.4.21.50 NH4+
-
 Achromobacter lyticus
3.4.21.50 phenylmethanesulfonyl fluoride
-
 Achromobacter lyticus
3.4.21.50 Rb+
-
 Achromobacter lyticus
3.4.21.50 Zn2+
-
 Achromobacter lyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.50 0.0019
-
 Trypsinogen
-
 Achromobacter lyticus
3.4.21.50 0.07
-
 N-benzoyl-L-Lys p-nitroanilide
-
 Achromobacter lyticus
3.4.21.50 0.08
-
 Lys-p-nitroanilide
-
 Achromobacter lyticus
3.4.21.50 0.091
-
 benzoyl-Lys methyl ester
-
 Achromobacter lyticus
3.4.21.50 0.1
-
 N-tosyl-L-Lys methyl ester
-
 Achromobacter lyticus
3.4.21.50 0.32
-
 benzoyl-Lys-NH2
-
 Achromobacter lyticus
3.4.21.50 1
-
 N-benzoyl-L-Orn methyl ester
-
 Achromobacter lyticus
3.4.21.50 3.09
-
 N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide
-
 Achromobacter lyticus
3.4.21.50 3.11
-
 N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide
-
 Achromobacter lyticus
3.4.21.50 35.7
-
 N-tosyl-L-Arg methyl ester
-
 Achromobacter lyticus
3.4.21.50 41
-
 N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide
-
 Achromobacter lyticus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.21.50 2-propanol 20%, 37°C, enzyme retains most of its activity Achromobacter lyticus
3.4.21.50 Ethanol 20%, 37°C, enzyme retains most of its activity Achromobacter lyticus
3.4.21.50 Methanol 20%, 37°C, enzyme retains most of its activity Achromobacter lyticus
3.4.21.50 SDS 0.1% w/v, in 40 mM Tris-HCl, pH 8, 20 min, 30°C, stable Achromobacter lyticus
3.4.21.50 urea 4 M, stable Achromobacter lyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.50 Achromobacter lyticus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.50 proteolytic modification the cloned gene of prepro-API is expressed in E. coli, pro-API is secreted into the periplasm and activated autocatalytically Achromobacter lyticus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.50
-
 Achromobacter lyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.50 additional information
-
-
 Achromobacter lyticus

Storage Stability

EC Number Storage Stability Organism
3.4.21.50 -20°C, 0.001-0.1 M Tris-HCl buffer, pH 6.0-10.9, at enzyme concentration higher than 0.1 mg/ml Achromobacter lyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.50 benzoyl-Lys methyl ester + H2O
-
 Achromobacter lyticus benzoyl-Lys + methanol
-
 ?
3.4.21.50 benzoyl-Lys-NH2 + H2O
-
 Achromobacter lyticus benzoyl-Lys + NH3
-
 ?
3.4.21.50 benzoyl-Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus benzoyl-Lys + p-nitroaniline
-
 ?
3.4.21.50 Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus Lys + p-nitroaniline
-
 ?
3.4.21.50 N-benzoyl-L-Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus N-benzoyl-L-Lys + p-nitroaniline
-
 ?
3.4.21.50 N-benzoyl-L-Orn methyl ester + H2O
-
 Achromobacter lyticus N-benzoyl-L-Orn + methanol
-
 ?
3.4.21.50 N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide + H2O
-
 Achromobacter lyticus N-tert-butyloxycarbonyl-Ala-Ala-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.50 N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide + H2O
-
 Achromobacter lyticus N-tert-butyloxycarbonyl-Ala-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.50 N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide + H2O
-
 Achromobacter lyticus N-tert-butyloxycarbonyl-Lys + 7-amino-4-methylcoumarin
-
 ?
3.4.21.50 N-tosyl-L-Arg methyl ester + H2O
-
 Achromobacter lyticus N-tosyl-L-Arg + methanol
-
 ?
3.4.21.50 N-tosyl-L-Lys methyl ester + H2O
-
 Achromobacter lyticus N-tosyl-L-Lys + methanol
-
 ?
3.4.21.50 Trypsinogen + H2O
-
 Achromobacter lyticus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.50 40
-
 stable Achromobacter lyticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.50 0.103
-
 Trypsinogen
-
 Achromobacter lyticus
3.4.21.50 0.14
-
 Lys-p-nitroanilide
-
 Achromobacter lyticus
3.4.21.50 0.86
-
 benzoyl-Lys-p-nitroanilide
-
 Achromobacter lyticus
3.4.21.50 1.54
-
 benzoyl-Lys-NH2
-
 Achromobacter lyticus
3.4.21.50 3.02
-
 N-tert-butyloxycarbonyl-Lys-4-methylcoumarin 7-amide
-
 Achromobacter lyticus
3.4.21.50 5.04
-
 N-tosyl-L-Arg methyl ester
-
 Achromobacter lyticus
3.4.21.50 10.4
-
 N-benzoyl-L-Orn methyl ester
-
 Achromobacter lyticus
3.4.21.50 32.9
-
 N-tert-butyloxycarbonyl-Ala-Lys-4-methylcoumarin 7-amide
-
 Achromobacter lyticus
3.4.21.50 49
-
 N-tert-butyloxycarbonyl-Ala-Ala-Lys-4-methylcoumarin 7-amide
-
 Achromobacter lyticus
3.4.21.50 225
-
 benzoyl-Lys methyl ester
-
 Achromobacter lyticus
3.4.21.50 570
-
 N-tosyl-L-Lys methyl ester
-
 Achromobacter lyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.50 7.8 8.2
-
 Achromobacter lyticus
3.4.21.50 8.5 10.7 caseinolytic activity Achromobacter lyticus
3.4.21.50 9 9.5
-
 Achromobacter lyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.50 4 10 stable Achromobacter lyticus