Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ornston, L.N.
    The conversion of catechol and protocatechuate to beta-ketoadipate by Pseudomonas putida (1966), J. Biol. Chem., 241, 3787-3794.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.5.1.2 CoCl2
-
 Pseudomonas putida
5.5.1.2 CuCl2
-
 Pseudomonas putida
5.5.1.2 NiCl2
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.24 0.012
-
 2-(5-oxo-4,5-dihydrofuran-2-yl)acetic acid
-
 Pseudomonas putida
5.5.1.2 0.075
-
 2-carboxy-2,5-dihydro-5-oxofuran-2-acetate
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.24 33000
-
-
 Pseudomonas putida
5.5.1.2 190000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.2 additional information Pseudomonas putida enzyme of the protocatechuate pathway ?
-
 ?
5.5.1.2 additional information Pseudomonas putida A.312 enzyme of the protocatechuate pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.24 Pseudomonas putida
-
 A.312
-
3.1.1.24 Pseudomonas putida A.312
-
 A.312
-
5.5.1.2 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.24 422fold Pseudomonas putida
5.5.1.2
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.24 717
-
-
 Pseudomonas putida
5.5.1.2 additional information
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.24 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O 3-oxoadipate enol-lactone Pseudomonas putida 3-oxoadipate
-
 ?
3.1.1.24 (5-oxo-4,5-dihydrofuran-2-yl)acetic acid + H2O 3-oxoadipate enol-lactone Pseudomonas putida A.312 3-oxoadipate
-
 ?
5.5.1.2 2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate i.e. beta-carboxy-cis,cis-muconate Pseudomonas putida cis-Butadiene-1,2,4-tricarboxylate i.e. gamma-carboxymuconolactone ?
5.5.1.2 2-Carboxy-2,5-dihydro-5-oxofuran-2-acetate i.e. beta-carboxy-cis,cis-muconate Pseudomonas putida A.312 cis-Butadiene-1,2,4-tricarboxylate i.e. gamma-carboxymuconolactone ?
5.5.1.2 additional information enzyme of the protocatechuate pathway Pseudomonas putida ?
-
 ?
5.5.1.2 additional information enzyme of the protocatechuate pathway Pseudomonas putida A.312 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.24 beta-ketoadipate enol-lactone hydrolase
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.24 55
-
 half-life, 3 min in 0.02 M sodium potassium phosphate buffer, pH 6.8, 0.005 mM Mg-EDTA Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.24 7.5 10 decrease of the activity is dependent on the buffer, the enzyme is less active in Tris-HCl than in potassium phosphate buffer at pH 7.5 Pseudomonas putida
5.5.1.2 6 7
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.5.1.2 5.5 9 pH 5.5: about 70% of maximal activity, pH 9.0: about 25% of maximal activity Pseudomonas putida