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Literature summary extracted from

  • Masaki, T.; Tanabe, M.; Nakamura, K.; Soejima, M.
    Studies on a new proteolytic enzyme from A chromobacter lyticus M497-1. I. Purification and some enzymatic properties (1981), Biochim. Biophys. Acta, 660, 44-50.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.50 Ba2+ competitive Achromobacter lyticus
3.4.21.50 Cs+ competitive Achromobacter lyticus
3.4.21.50 diisopropylfluorophosphate
-
 Achromobacter lyticus
3.4.21.50 K+ competitive Achromobacter lyticus
3.4.21.50 Li+ competitive Achromobacter lyticus
3.4.21.50 Na+ competitive Achromobacter lyticus
3.4.21.50 Nalpha-p-tosyl-L-Lys-chloromethylketone
-
 Achromobacter lyticus
3.4.21.50 NH4+ competitive Achromobacter lyticus
3.4.21.50 phenylmethanesulfonyl fluoride
-
 Achromobacter lyticus
3.4.21.50 Rb+ competitive Achromobacter lyticus
3.4.21.50 Zn2+
-
 Achromobacter lyticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.50 extracellular
-
 Achromobacter lyticus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.50 30000
-
 1 * 30000, SDS-PAGE Achromobacter lyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.50 Achromobacter lyticus
-
 M497-1
-
3.4.21.50 Achromobacter lyticus M497-1
-
 M497-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.50
-
 Achromobacter lyticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.50 culture filtrate
-
 Achromobacter lyticus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.50 casein + H2O
-
 Achromobacter lyticus ?
-
 ?
3.4.21.50 casein + H2O
-
 Achromobacter lyticus M497-1 ?
-
 ?
3.4.21.50 additional information the enzyme cleaves only the Lys-X bond Achromobacter lyticus ?
-
 ?
3.4.21.50 additional information the enzyme cleaves only the Lys-X bond Achromobacter lyticus M497-1 ?
-
 ?
3.4.21.50 N-benzoyl-L-Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus N-benzoyl-L-Lys + p-nitroaniline
-
 ?
3.4.21.50 N-benzoyl-L-Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus M497-1 N-benzoyl-L-Lys + p-nitroaniline
-
 ?
3.4.21.50 N-tosyl-L-Lys methyl ester + H2O
-
 Achromobacter lyticus N-tosyl-L-Lys + methanol
-
 ?
3.4.21.50 N-tosyl-L-Lys methyl ester + H2O
-
 Achromobacter lyticus M497-1 N-tosyl-L-Lys + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.50 monomer 1 * 30000, SDS-PAGE Achromobacter lyticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.50 50
-
 amidolytic activity, esterolytic activity and caseinolytic activity Achromobacter lyticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.50 45
-
 pH 9.0, 30 min, stable up to Achromobacter lyticus
3.4.21.50 60
-
 30 min, complete loss of activity Achromobacter lyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.50 7.5 8.2 esterolytic activity Achromobacter lyticus
3.4.21.50 7.8 8.2 esterolytic activity Achromobacter lyticus
3.4.21.50 9 9.5 amidolytic activity Achromobacter lyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.50 4 11 4°C, 24 h, stable Achromobacter lyticus