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Literature summary extracted from

  • Masaki, T.; Suzuki, H.; Soejima, M.
    Purification and some properties of Achromobacter protease Ia from Achromobacter lyticus M497-1 (1986), Agric. Biol. Chem., 50, 3087-3091.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.4.21.50 7 M urea, pH 8.0, 30°C, 20 min, 30% loss of activity Achromobacter lyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.50 Zn2+
-
 Achromobacter lyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.50 0.07
-
 N-benzoyl-L-Lys p-nitroanilide protease I Achromobacter lyticus
3.4.21.50 0.08
-
 Lys-p-nitroanilide protease I Achromobacter lyticus
3.4.21.50 0.083
-
 N-tosyl-L-Lys methyl ester protease Ia Achromobacter lyticus
3.4.21.50 0.09
-
 N-benzoyl-L-Lys p-nitroanilide protease Ia Achromobacter lyticus
3.4.21.50 0.1
-
 N-tosyl-L-Lys methyl ester protease I Achromobacter lyticus
3.4.21.50 0.11
-
 Lys-p-nitroanilide protease Ia Achromobacter lyticus
3.4.21.50 0.63
-
 N-benzoyl-L-Orn methyl ester protease Ia Achromobacter lyticus
3.4.21.50 1
-
 N-benzoyl-L-Orn methyl ester protease I Achromobacter lyticus
3.4.21.50 2.5
-
 N-tosyl-L-Arg methyl ester protease Ia Achromobacter lyticus
3.4.21.50 35.7
-
 N-tosyl-L-Arg methyl ester protease I Achromobacter lyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.50 27000
-
 gel filtration Achromobacter lyticus
3.4.21.50 30000
-
 gel filtration Achromobacter lyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.50 Achromobacter lyticus
-
 M497-1
-
3.4.21.50 Achromobacter lyticus
-
 protease I and protease Ia
-
3.4.21.50 Achromobacter lyticus M497-1
-
 M497-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.50 protease Ia Achromobacter lyticus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.50 culture filtrate
-
 Achromobacter lyticus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.50 additional information
-
-
 Achromobacter lyticus

Storage Stability

EC Number Storage Stability Organism
3.4.21.50 4°C, pH 5.0-11.0, 24 h, stable Achromobacter lyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.50 B-chain of insulin + H2O protease I1 cleaves Lys29-Ala30 bond Achromobacter lyticus ?
-
 ?
3.4.21.50 B-chain of insulin + H2O protease I1 cleaves Lys29-Ala30 bond Achromobacter lyticus M497-1 ?
-
 ?
3.4.21.50 Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus Lys + p-nitroaniline
-
 ?
3.4.21.50 Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus M497-1 Lys + p-nitroaniline
-
 ?
3.4.21.50 additional information no hydrolysis of: N-benzoyl-L-Arg p-nitroanilide and Arg-p-nitroanilide Achromobacter lyticus ?
-
 ?
3.4.21.50 additional information no hydrolysis of: N-benzoyl-L-Arg p-nitroanilide and Arg-p-nitroanilide Achromobacter lyticus M497-1 ?
-
 ?
3.4.21.50 N-benzoyl-L-Lys-p-nitroanilide + H2O
-
 Achromobacter lyticus N-benzoyl-L-Lys + p-nitroaniline
-
 ?
3.4.21.50 N-benzoyl-L-Orn methyl ester + H2O
-
 Achromobacter lyticus N-benzoyl-L-Orn + methanol
-
 ?
3.4.21.50 N-tosyl-L-Arg methyl ester + H2O
-
 Achromobacter lyticus N-tosyl-L-Arg + methanol
-
 ?
3.4.21.50 N-tosyl-L-Arg methyl ester + H2O
-
 Achromobacter lyticus M497-1 N-tosyl-L-Arg + methanol
-
 ?
3.4.21.50 N-tosyl-L-Lys methyl ester + H2O
-
 Achromobacter lyticus N-tosyl-L-Lys + methanol
-
 ?
3.4.21.50 N-tosyl-L-Lys methyl ester + H2O
-
 Achromobacter lyticus M497-1 N-tosyl-L-Lys + methanol
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.50 40
-
 20 min, stable up to Achromobacter lyticus
3.4.21.50 60
-
 20 min, complete loss of activity Achromobacter lyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.50 7.8 8.2 esterolytic activity Achromobacter lyticus
3.4.21.50 8.8
-
 amidolytic activity Achromobacter lyticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.50 5 11 4°C, 24 h, stable Achromobacter lyticus