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Literature summary extracted from

  • Mazur, P.; Piecken, W.A.; Budihas, S.R.; Williams, S.E.; Wong, S.; Kozarich, J.W.
    cis,cis-Muconate lactonizing enzyme from Trichosporon cutaneum: evidence for a novel class of cycloisomerases in eucaryotes (1994), Biochemistry, 33, 1961-1970.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.1
-
 Cutaneotrichosporon cutaneum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.5.1.1 43000
-
 4 * 43000, SDS-PAGE Cutaneotrichosporon cutaneum
5.5.1.1 169000
-
 gel filtration Cutaneotrichosporon cutaneum

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.1 Cutaneotrichosporon cutaneum
-
-
-
5.5.1.7 Pseudomonas sp.
-
 B13
-
5.5.1.7 Pseudomonas sp. B13
-
 B13
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.1
-
 Cutaneotrichosporon cutaneum

Reaction

EC Number Reaction Comment Organism Reaction ID
5.5.1.1 (+)-muconolactone = cis,cis-muconate syn lactonization Cutaneotrichosporon cutaneum
a
5.5.1.7 (2R)-2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate syn cycloisomerization Pseudomonas sp.
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5.1.1 18.2
-
-
 Cutaneotrichosporon cutaneum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.1 3-Bromo-cis,cis-muconate at 9% of the activity relative to 3-methyl-cis,cis-muconate Cutaneotrichosporon cutaneum ?
-
 ?
5.5.1.1 3-Chloro-cis,cis-muconate at 11% of the activity relative to 3-methyl-cis,cis-muconate Cutaneotrichosporon cutaneum 5-Chloromuconolactone
-
 ?
5.5.1.1 3-Fluoro-cis,cis-muconate at 17% of the activity relative to 3-methyl-cis,cis-muconate Cutaneotrichosporon cutaneum (+)-4-Fluoromuconolactone
-
 ?
5.5.1.1 3-Methyl-cis,cis-muconate
-
 Cutaneotrichosporon cutaneum beta-Methylmuconolactone
-
 ?
5.5.1.1 cis,cis-Muconate
-
 Cutaneotrichosporon cutaneum (+)-Muconolactone i.e. 5-oxo-2,5-dihydrofuran-2-acetate, , (4S)-muconolactone ?
5.5.1.7 3-Bromo-cis,cis-muconate
-
 Pseudomonas sp. 5-bromomuconolactone
-
 ?
5.5.1.7 3-Bromo-cis,cis-muconate
-
 Pseudomonas sp. B13 5-bromomuconolactone
-
 ?
5.5.1.7 3-Chloro-cis,cis-muconate
-
 Pseudomonas sp. cis-4-Carboxymethylenebut-2-en-4-olide corresponding (E)-dienenlactone ?
5.5.1.7 3-Chloro-cis,cis-muconate
-
 Pseudomonas sp. B13 cis-4-Carboxymethylenebut-2-en-4-olide corresponding (E)-dienenlactone ?

Subunits

EC Number Subunits Comment Organism
5.5.1.1 tetramer 4 * 43000, SDS-PAGE Cutaneotrichosporon cutaneum