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Literature summary extracted from

  • Diaz-Maurino, T.; Nieto, M.; Perkins, H.R.
    Membrane-bound DD-carboxypeptidases from Bacillus megaterium KM (1974), Biochem. J., 134, 391-402.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.8 acetyl-Gly3-D-Ala-D-Asp
-
 Priestia megaterium
3.4.17.8 carbenicillin
-
 Priestia megaterium
3.4.17.8 Cu2+
-
 Priestia megaterium
3.4.17.8 Hg2+
-
 Priestia megaterium
3.4.17.8 Methicillin
-
 Priestia megaterium
3.4.17.8 penicillin G
-
 Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.8 0.33
-
 Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
 Priestia megaterium
3.4.17.8 0.4
-
 UDP-N-acetylmuramoyl-L-Ala-gamma-D-Glu-L-mesodiaminopimelyl-D-Ala-D-Ala
-
 Priestia megaterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.8 Ca2+ activation Priestia megaterium
3.4.17.8 Co2+ activation Priestia megaterium
3.4.17.8 Mg2+ activation Priestia megaterium
3.4.17.8 Mn2+ activation Priestia megaterium
3.4.17.8 Zn2+ activation Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.17.8 UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine + H2O Priestia megaterium
-
 ?
-
 ?
3.4.17.8 UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine + H2O Priestia megaterium KM / ATCC 13632
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.8 Priestia megaterium
-
 KM
-
3.4.17.8 Priestia megaterium KM / ATCC 13632
-
 KM
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.8 culture filtrate
-
 Priestia megaterium
-

Storage Stability

EC Number Storage Stability Organism
3.4.17.8 -20°C, membrane preparation, pH 7.8 or 5.0, 1 year Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.8 additional information influence of residue preceeding C-terminal D-Ala-D-Ala on rate of hydrolysis Priestia megaterium ?
-
 ?
3.4.17.8 additional information influence of residue preceeding C-terminal D-Ala-D-Ala on rate of hydrolysis Priestia megaterium KM / ATCC 13632 ?
-
 ?
3.4.17.8 Nalpha, Nepsilon-diacetyl L-Lys-D-Ala-Gly + H2O
-
 Priestia megaterium Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + Gly
-
 ?
3.4.17.8 Nalpha, Nepsilon-diacetyl L-Lys-D-Ala-Gly + H2O
-
 Priestia megaterium KM / ATCC 13632 Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + Gly
-
 ?
3.4.17.8 Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Priestia megaterium Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
-
 ?
3.4.17.8 Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
-
 Priestia megaterium KM / ATCC 13632 Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
-
 ?
3.4.17.8 UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine + H2O
-
 Priestia megaterium ?
-
 ?
3.4.17.8 UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine + H2O
-
 Priestia megaterium KM / ATCC 13632 ?
-
 ?
3.4.17.8 UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-L-mesodiaminopimelyl-D-Ala-D-Ala + H2O
-
 Priestia megaterium UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-L-mesodiaminopimelyl-D-Ala + D-Ala
-
 ?
3.4.17.8 UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-L-mesodiaminopimelyl-D-Ala-D-Ala + H2O
-
 Priestia megaterium KM / ATCC 13632 UDP-N-acetylmuramyl-L-Ala-gamma-D-Glu-L-mesodiaminopimelyl-D-Ala + D-Ala
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.17.8 35
-
 up to Priestia megaterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.8 5.2
-
-
 Priestia megaterium