Literature summary extracted from

Marinkovic, D.V.; Marinkovic, J.N.; Erd�s, E.G.; Robinson, C.J.G.
Purification of carboxypeptidase B from human pancreas (1977), Biochem. J., 163, 253-260.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.4.17.2	6-amino-n-hexanoic acid	-	Homo sapiens
3.4.17.2	Co2+	hydrolysis of hippuryl-L-Arg	Homo sapiens
3.4.17.2	EDTA	partial	Homo sapiens
3.4.17.2	SDS	-	Homo sapiens
3.4.17.2	Urea	-	Homo sapiens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.4.17.2	0.05	-	hippuryl-L-Arg	carboxypeptidase B I	Homo sapiens
3.4.17.2	0.071	-	hippuryl-L-Arg	carboxypeptidase B II	Homo sapiens
3.4.17.2	0.277	-	hippuryl-Arg	carboxypeptidase B I	Homo sapiens
3.4.17.2	0.31	-	hippuryl-Arg	carboxypeptidase B II	Homo sapiens

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
3.4.17.2	Co2+	CoCl2, accelerates hydrolysis of hippuryl-L-Arg and hippuryl-Lys	Homo sapiens

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.4.17.2	32210	-	carboxypeptidase B II, gel filtration	Homo sapiens
3.4.17.2	32920	-	carboxypeptidase B I, gel filtration	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.17.2	Homo sapiens	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.17.2	-	Homo sapiens

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.4.17.2	pancreas	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.4.17.2	862	-	carboxypeptidase B I	Homo sapiens
3.4.17.2	1192	-	carboxypeptidase B II	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.17.2	hippuryl-L-Arg	-	Homo sapiens	hippuric acid + L-Arg	-	?
3.4.17.2	hippuryl-Lys + H2O	peptidase activity	Homo sapiens	hippuric acid + Lys	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
3.4.17.2	7	-	cleavage of hippuryl-L-Arg	Homo sapiens
3.4.17.2	10	-	hydrolysis of hippuryl-L-Arg	Homo sapiens

pH Range

EC Number	pH Minimum	pH Maximum	Comment	Organism
3.4.17.2	6	10	pH 6: about 60% of maximal activity with carboxypeptidase B I, about 50% of maximal activity with carboxypeptidase B II, pH 10: about 70% of maximal activity with carboxypeptidase B I, about 60% of maximal activity with carboxypeptidase B II, cleavage of hippuryl-L-Arg	Homo sapiens
3.4.17.2	7	10.5	pH 7: about 45% of maximal activity with carboxypeptidase B I, about 30% of maximal activity with carboxypeptidase B II, pH: 10.5: about 90% of maximal activity with carboxypeptidase B I, about 80% of maximal activity with carboxypeptidase B II, hydrolysis of hippuryl-L-Arg	Homo sapiens

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Release 2023.1 (January 2023)