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Literature summary extracted from

  • Folk, J.E.
    Carboxypeptidase B (porcine pancreas) (1970), Methods Enzymol., 19, 504-508.
No PubMed abstract available

General Stability

EC Number General Stability Organism
3.4.17.2 25-45% loss of activity after lyophilization Bos taurus
3.4.17.2 stable to several thawings and refreezings Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.2 Zinc contains 1 gatom of zinc per mol of enzyme Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.2 34300
-
 calculation from sedimentation and diffusion data Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.2 pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.2 175
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
3.4.17.2 frozen, stable for up to 1 year Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.2 additional information hydrolysis of peptide bonds of Lys, Arg, Arn, homoarginine and S-(beta-aminoethyl)cysteine. Esterase activity towards carboxy terminal Arg Bos taurus ?
-
 ?
3.4.17.2 N-benzoyl-Gly-L-Arg + H2O
-
 Bos taurus N-benzoyl-Gly + L-Arg
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.2 7 9
-
 Bos taurus