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Literature summary extracted from
- Preparation, characterization and application of immobilized carboxypeptidase A (1999), Enzyme Microb. Technol., 25, 73-79.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.17.1 | synthesis | immobilization for synthetic use | Bos taurus |
| 3.4.17.1 | synthesis | useful tools for peptide biosynthesis in non-conventional media, considered | Bos taurus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.17.1 | hydrophobic environment of immobilized enzyme enhances conformational stability | Bos taurus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.1 | 1.07 | - |
hippuryl-L-Phe | soluble enzyme | Bos taurus |  |
| 3.4.17.1 | 1.65 | - |
hippuryl-L-Phe | immobilized enzyme | Bos taurus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.17.1 | Bos taurus | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | additional information | - |
comparison of activities of enzyme immobilized on different supports, highest activity for polyacrylamide bead support | Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.1 | hippuryl-L-Phe + H2O | - |
Bos taurus | hippuric acid + L-Phe | - |
ir | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | 30 | 35 | soluble enzyme | Bos taurus |
| 3.4.17.1 | 50 | 55 | immobilized enzyme | Bos taurus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | 55 | - |
35% activity after 30 min of immobilized enzyme, no activity of soluble enzyme | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | 7.5 | - |
assay at | Bos taurus |
| 3.4.17.1 | 7.5 | 8 | identical for soluble and immobilized enzymes | Bos taurus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.1 | 8.5 | - |
quick inactivation of soluble enzyme, no inactivation of immobilized enzyme | Bos taurus |
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