Literature summary extracted from
Lee, K.J.; Kim, D.H.
Design of mechanism-based carboxypeptidase A inactivators on the basis of the X-ray crystal structure and catalytic reaction pathway (1998), Bioorg. Med. Chem., 6, 1613-1622.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.4.17.1 |
medicine |
design of mechanism-based, specific inhibitors to cure disease caused by enzyme overfunction |
Bos taurus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.4.17.1 |
structural analysis of enzyme-GlyTyr complexes, design of mechanism-based inactivators based thereon |
Bos taurus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.17.1 |
N-(hydroxyacetyl)-Phe |
irreversible inactivation, Kinact/Ki: 71 M-1s-1 |
Bos taurus |
|
3.4.17.1 |
O-(hydroxyacetyl)-beta-phenyllactate |
irreversible inactivation, Kinact/Ki: 57 M-1s-1 |
Bos taurus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.17.1 |
Bos taurus |
- |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.4.17.1 |
release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro |
mechanism |
Bos taurus |
|
3.4.17.1 |
release of a C-terminal amino acid, but little or no action with -Asp, -Glu, -Arg, -Lys or -Pro |
evidence of general base pathway |
Bos taurus |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.17.1 |
Gly-L-Tyr + H2O |
low activity |
Bos taurus |
Gly + L-Tyr |
- |
ir |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.4.17.1 |
25 |
- |
assay at |
Bos taurus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.4.17.1 |
7.5 |
- |
assay at |
Bos taurus |