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Literature summary extracted from

  • Bodwell, J.E.; Meyer, W.L.
    Purification and characterization of carboxypeptidase A from rat skeletal muscle (1981), Biochemistry, 20, 2767-2777.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.17.1 generally unstable at all stages of purification Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.17.1 1,10-phenanthroline
-
 Rattus norvegicus
3.4.17.1 3-Phenylpropionic acid Ki: 0.81 mM, noncompetitive Rattus norvegicus
3.4.17.1 Chelating agents
-
 Rattus norvegicus
3.4.17.1 Hydroxyquinoline sulfonate
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.17.1 0.11
-
 hippuryl-L-phenyllactate
-
 Rattus norvegicus
3.4.17.1 2.7
-
 hippuryl-L-Phe
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.17.1 Zn2+ metalloenzyme Rattus norvegicus
3.4.17.1 Zn2+ 1 mol Zn per mol of enzyme Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.17.1 37800
-
 SDS-PAGE, component II Rattus norvegicus
3.4.17.1 39300
-
 SDS-PAGE, component I Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.17.1 Rattus norvegicus
-
 2 components: I, II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.17.1
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.17.1 skeletal muscle mast cell Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.17.1 additional information
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
3.4.17.1 -20°C, NaCl-HEPES, pH 7.5, purified enzyme, 1 month, 10% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.17.1 hippuryl-DL-phenyllactate + H2O
-
 Rattus norvegicus hippuric acid + DL-phenyllactate
-
 ir
3.4.17.1 hippuryl-L-Phe + H2O
-
 Rattus norvegicus hippuric acid + L-Phe
-
 ir
3.4.17.1 hippuryl-L-phenyllactate + H2O
-
 Rattus norvegicus hippuric acid + L-phenyllactate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.17.1 21
-
 assay at Rattus norvegicus
3.4.17.1 30
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.17.1 30
-
 10 min, 50% loss of activity Rattus norvegicus
3.4.17.1 50
-
 10 min, 50% loss of activity Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.17.1 40
-
 hippuryl-L-Phe
-
 Rattus norvegicus
3.4.17.1 933
-
 hippuryl-L-phenyllactate
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.17.1 7 9
-
 Rattus norvegicus
3.4.17.1 7.5
-
 assay at Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.17.1 5 10 pH 5: about 20% of maximum activity, pH 10: about 50% of maximum activity Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.17.1 6 9
-
 Rattus norvegicus