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Literature summary extracted from

  • Miyakawa, H.; Hashimoto, I.; Nakamura, T.; Ishibashi, N.; Shimamura, S.; Igoshi, K.
    Purification and characterization of an X-prolyl dipeptidyl aminopeptidase from Lactobacillus helveticus LHE-511 (1994), Milchwissenschaft, 49, 670-673.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.5 1,10-phenanthroline
-
 Lactobacillus helveticus
3.4.14.5 Cu2+
-
 Lactobacillus helveticus
3.4.14.5 diisopropyl fluorophosphate
-
 Lactobacillus helveticus
3.4.14.5 dithiothreitol
-
 Lactobacillus helveticus
3.4.14.5 EDTA
-
 Lactobacillus helveticus
3.4.14.5 Hg2+
-
 Lactobacillus helveticus
3.4.14.5 NEM
-
 Lactobacillus helveticus
3.4.14.5 PCMB
-
 Lactobacillus helveticus
3.4.14.5 phenylmethylsulfonyl fluoride
-
 Lactobacillus helveticus
3.4.14.11 Cu2+
-
 Lactobacillus helveticus
3.4.14.11 diisopropylfluorophosphate
-
 Lactobacillus helveticus
3.4.14.11 Hg2+
-
 Lactobacillus helveticus
3.4.14.11 PCMB
-
 Lactobacillus helveticus
3.4.14.11 phenylmethylsulfonyl fluoride
-
 Lactobacillus helveticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.14.11 Fe2+ weak activation Lactobacillus helveticus
3.4.14.11 Mg2+ weak activation Lactobacillus helveticus
3.4.14.11 Zn2+ weak activation Lactobacillus helveticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.5 87000
-
 1 * 87000, SDS-PAGE Lactobacillus helveticus
3.4.14.5 93000
-
 gel filtration Lactobacillus helveticus
3.4.14.11 87000
-
 1 * 87000, SDS-PAGE Lactobacillus helveticus
3.4.14.11 93000
-
 gel filtration Lactobacillus helveticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Lactobacillus helveticus
-
 LHE-511
-
3.4.14.5 Lactobacillus helveticus LHE-511
-
 LHE-511
-
3.4.14.11 Lactobacillus helveticus
-
 LHE-511
-
3.4.14.11 Lactobacillus helveticus LHE-511
-
 LHE-511
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.5
-
 Lactobacillus helveticus
3.4.14.11
-
 Lactobacillus helveticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.5 20.2
-
-
 Lactobacillus helveticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Ala-Ala-4-nitroanilide + H2O
-
 Lactobacillus helveticus Ala-Ala + 4-nitroaniline
-
 ?
3.4.14.5 Ala-Ala-4-nitroanilide + H2O
-
 Lactobacillus helveticus LHE-511 Ala-Ala + 4-nitroaniline
-
 ?
3.4.14.5 Ala-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus Ala-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Ala-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus LHE-511 Ala-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Arg-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus Arg-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Arg-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus LHE-511 Arg-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Arg-Pro-Pro + H2O
-
 Lactobacillus helveticus ?
-
 ?
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Gly-Pro-4-nitroanilide + H2O
-
 Lactobacillus helveticus LHE-511 Gly-Pro + 4-nitroaniline
-
 ?
3.4.14.5 Gly-Pro-Arg + H2O
-
 Lactobacillus helveticus Gly-Pro + Arg
-
 ?
3.4.14.5 Gly-Pro-Arg + H2O
-
 Lactobacillus helveticus LHE-511 Gly-Pro + Arg
-
 ?
3.4.14.5 Ile-Pro-Ile + H2O
-
 Lactobacillus helveticus ?
-
 ?
3.4.14.5 Val-Pro-Leu + H2O
-
 Lactobacillus helveticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.5 monomer 1 * 87000, SDS-PAGE Lactobacillus helveticus
3.4.14.11 monomer 1 * 87000, SDS-PAGE Lactobacillus helveticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.5 50
-
-
 Lactobacillus helveticus
3.4.14.11 50
-
-
 Lactobacillus helveticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.14.5 60
-
 30 min, about 40% loss of activity Lactobacillus helveticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 6.5
-
-
 Lactobacillus helveticus
3.4.14.11 6.5
-
-
 Lactobacillus helveticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.14.5 5 8 pH 5.0: about 50% of maximal activity, pH 8.0: about 60% of maximal activity Lactobacillus helveticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.14.5 4.5 7.5 37°C, 60 min, stable Lactobacillus helveticus