Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Habibi-Najafi, M.B.; Lee, B.H.
    Purification and characterization of X-prolyl dipeptidyl peptidase from Lactobacillus casei subsp. casei LLG (1994), Appl. Microbiol. Biotechnol., 42, 280-286.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.5 diisopropyl fluorophosphate
-
 Lactococcus lactis
3.4.14.5 Fe2+ 1 mM, 13% inhibition Lactococcus lactis
3.4.14.5 Hg2+
-
 Lactococcus lactis
3.4.14.5 phenylmethylsulfonyl fluoride
-
 Lactococcus lactis
3.4.14.11 Cu2+ weak Lacticaseibacillus casei
3.4.14.11 diisopropylfluorophosphate
-
 Lacticaseibacillus casei
3.4.14.11 Fe3+ weak Lacticaseibacillus casei
3.4.14.11 Hg2+
-
 Lacticaseibacillus casei
3.4.14.11 phenylmethylsulfonyl fluoride
-
 Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.11 0.2
-
 Gly-Pro-7-amido-4-methylcoumarin
-
 Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.11 79000
-
 x * 79000, native PAGE and SDS-PAGE Lacticaseibacillus casei

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Lactococcus lactis
-
 subsp. casei LLG
-
3.4.14.11 Lacticaseibacillus casei
-
 LLG
-
3.4.14.11 Lacticaseibacillus casei
-
 subsp. casei
-
3.4.14.11 Lacticaseibacillus casei LLG
-
 LLG
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.5
-
 Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.5 additional information
-
-
 Lactococcus lactis
3.4.14.11 71.12
-
-
 Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Gly-Pro-4-methylcoumarin 7-amide + H2O
-
 Lactococcus lactis Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.5 Lys-Pro-4-methoxy-2-naphthylamide + H2O
-
 Lactococcus lactis Lys-Pro + 4-methoxy-2-naphthylamine
-
 ?
3.4.14.11 Gly-Pro-7-amido-4-methylcoumarin + H2O
-
 Lacticaseibacillus casei Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.14.11 Gly-Pro-7-amido-4-methylcoumarin + H2O
-
 Lacticaseibacillus casei LLG Gly-Pro + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.11 ? x * 79000, native PAGE and SDS-PAGE Lacticaseibacillus casei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.5 50
-
-
 Lactococcus lactis
3.4.14.11 50
-
-
 Lacticaseibacillus casei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.14.5 15 55 15°C: about 30% of maximal activity, 55°C: about 40% of maximal activity Lactococcus lactis
3.4.14.11 20 55 20°C: about 30% of activity maximum, 55°C: about 40% of activity maximum Lacticaseibacillus casei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 7
-
 two optima at pH 7.0 and at pH 9.5 Lactococcus lactis
3.4.14.5 9.5
-
 two optima at pH 7.0 and at pH 9.5 Lactococcus lactis
3.4.14.11 7
-
 and a second optimum at pH 9.5 Lacticaseibacillus casei
3.4.14.11 9.5
-
 and a second pH-optimum at pH 7.0 Lacticaseibacillus casei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.14.5 5 10.5 pH 5.0: about 55% of maximal activity, pH 10.5: about 30% of maximal activity Lactococcus lactis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.14.5 9
-
 12 h, stable below Lactococcus lactis
3.4.14.5 10
-
 1 h, stable below Lactococcus lactis
3.4.14.11 5.5 9 12 h, stable Lacticaseibacillus casei