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Literature summary extracted from

  • Bella, A.M.; Erickson, R.H.; Kim, Y.S.
    Rat intestinal brush border membrane dipeptidyl-aminopeptidase IV: kinetic properties and substrate specificities of the purified enzyme (1982), Arch. Biochem. Biophys., 218, 156-162.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.14.5 complete loss of activity when frozen in imidazole buffer Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.14.5 Ala-Pro
-
 Rattus norvegicus
3.4.14.5 Cd2+
-
 Rattus norvegicus
3.4.14.5 Hg2+
-
 Rattus norvegicus
3.4.14.5 L-His-L-Ala
-
 Rattus norvegicus
3.4.14.5 L-Leu-L-Ala
-
 Rattus norvegicus
3.4.14.5 L-Leu-L-Leu
-
 Rattus norvegicus
3.4.14.5 L-Leu-L-Pro
-
 Rattus norvegicus
3.4.14.5 L-Lys-L-Ala
-
 Rattus norvegicus
3.4.14.5 L-Met-L-Ala
-
 Rattus norvegicus
3.4.14.5 L-Met-L-Pro
-
 Rattus norvegicus
3.4.14.5 L-Phe-L-Ala
-
 Rattus norvegicus
3.4.14.5 L-Phe-L-Pro
-
 Rattus norvegicus
3.4.14.5 L-Val-L-Ala
-
 Rattus norvegicus
3.4.14.5 L-Val-L-Pro
-
 Rattus norvegicus
3.4.14.5 L-Val-L-Val
-
 Rattus norvegicus
3.4.14.5 Pro-Ala hydrolysis of L-Pro-L-Ala Rattus norvegicus
3.4.14.5 Zn2+
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.5 0.12
-
 L-Leu-L-Pro-Gly-Gly
-
 Rattus norvegicus
3.4.14.5 0.17
-
 L-Ala-L-Pro-Gly
-
 Rattus norvegicus
3.4.14.5 0.2
-
 L-Lys-L-Ala-2-naphthylamide
-
 Rattus norvegicus
3.4.14.5 0.24
-
 L-Leu-L-Ala-2-naphthylamide
-
 Rattus norvegicus
3.4.14.5 0.26
-
 Gly-L-Pro-2-naphthylamide
-
 Rattus norvegicus
3.4.14.5 0.28
-
 Gly-L-Pro-Gly-Gly
-
 Rattus norvegicus
3.4.14.5 0.29
-
 Hly-Pro-2-naphthylamide
-
 Rattus norvegicus
3.4.14.5 0.31
-
 Gly-L-Pro-L-Ala
-
 Rattus norvegicus
3.4.14.5 0.35
-
 L-Leu-L-Leu-L-Leu
-
 Rattus norvegicus
3.4.14.5 0.36
-
 L-Phe-L-Pro-2-naphthylamide
-
 Rattus norvegicus
3.4.14.5 1.25
-
 L-Leu-L-Leu-Gly
-
 Rattus norvegicus
3.4.14.5 2.5
-
 L-Ala-L-Ala-L-Ala
-
 Rattus norvegicus
3.4.14.5 5
-
 Gly-L-Ala-2-naphthylamide
-
 Rattus norvegicus
3.4.14.5 7.5
-
 L-Ala-L-Ala-2-naphthylamide
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.14.5 brush border
-
 Rattus norvegicus 5903
-

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.5 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.14.5 intestine
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.4.14.5 -20°C, 50 mM ammonium acetate buffer, pH 7, stable for several months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.5 Ala-Ala-2-naphthylamide + H2O
-
 Rattus norvegicus Ala-Ala + 2-naphthylamine
-
 ?
3.4.14.5 Ala-Ala-Ala + H2O L-Ala-L-Ala-L-Ala Rattus norvegicus Ala-Ala + Ala
-
 ?
3.4.14.5 Ala-Pro-Gly + H2O
-
 Rattus norvegicus Ala-Pro + Gly
-
 ?
3.4.14.5 Gly-L-Ala-2-naphthylamide + H2O
-
 Rattus norvegicus Gly-L-Ala + 2-naphthylamine
-
 ?
3.4.14.5 Gly-L-Pro-Gly-Gly + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.5 Gly-L-Pro-L-Ala + H2O
-
 Rattus norvegicus Gly-L-Pro + L-Ala
-
 ?
3.4.14.5 Gly-Pro-2-naphthylamide + H2O L-Gly-Pro-2-naphthylamide Rattus norvegicus Gly-Pro + 2-naphthylamine
-
 ?
3.4.14.5 Hly-Pro-2-naphthylamide
-
 Rattus norvegicus ?
-
 ?
3.4.14.5 L-Leu-L-Ala-2-naphthylamide + H2O
-
 Rattus norvegicus L-Leu-L-Ala + 2-naphthylamine
-
 ?
3.4.14.5 L-Leu-L-Leu-Gly + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.5 L-Leu-L-Leu-L-Leu + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.5 L-Leu-L-Pro-Gly-Gly + H2O
-
 Rattus norvegicus ?
-
 ?
3.4.14.5 L-Lys-L-Ala-2-naphthylamide + H2O
-
 Rattus norvegicus L-Lys-L-Ala + 2-naphthylamine
-
 ?
3.4.14.5 additional information peptides with proline penultimate to the amino terminus are more favored substrates while those substrates having Leu at both the penultimate position and amino terminus Leu-Leu Rattus norvegicus ?
-
 ?
3.4.14.5 Phe-Pro-2-naphthylamide + H2O
-
 Rattus norvegicus Phe-Pro + 2-naphthylamine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.14.5 40
-
 stable up to Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.5 1.5
-
 L-Leu-L-Leu-Gly
-
 Rattus norvegicus
3.4.14.5 2.7
-
 L-Leu-L-Leu-L-Leu
-
 Rattus norvegicus
3.4.14.5 5.6
-
 Gly-L-Ala-2-naphthylamide
-
 Rattus norvegicus
3.4.14.5 6.3
-
 L-Lys-L-Ala-2-naphthylamide
-
 Rattus norvegicus
3.4.14.5 11.2
-
 L-Leu-L-Ala-2-naphthylamide
-
 Rattus norvegicus
3.4.14.5 15.1
-
 L-Ala-L-Ala-L-Ala
-
 Rattus norvegicus
3.4.14.5 31.3
-
 Gly-L-Pro-Gly-Gly
-
 Rattus norvegicus
3.4.14.5 46.2
-
 L-Ala-L-Pro-Gly
-
 Rattus norvegicus
3.4.14.5 52.3
-
 L-Ala-L-Ala-2-naphthylamide
-
 Rattus norvegicus
3.4.14.5 82.3
-
 Gly-L-Pro-L-Ala
-
 Rattus norvegicus
3.4.14.5 82.8
-
 L-Leu-L-Pro-Gly-Gly
-
 Rattus norvegicus
3.4.14.5 128
-
 Gly-L-Pro-2-naphthylamide
-
 Rattus norvegicus
3.4.14.5 990
-
 L-Phe-L-Pro-2-naphthylamide
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.14.5 8.2 8.6 ,hydrolysis of Gly-Pro-2-naphthylamide Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.14.5 6.5 9.5 pH 6.5: about 60% of maximal activity, pH 9.5: about 45% of maximal activity Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.14.5 6
-
 rapid inactivation below Rattus norvegicus
3.4.14.5 11
-
 stable up to Rattus norvegicus