BRENDA - Enzyme Database

Purification and characterization of enkephalinase B from rat brain membrane

Inaoka, Y.; Tamaoki, H.; Biochim. Biophys. Acta 925, 27-35 (1987) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.14.4
1,10-phenanthroline
-
Rattus norvegicus
3.4.14.4
EDTA
-
Rattus norvegicus
3.4.14.4
p-hydroxymercuribenzoate
-
Rattus norvegicus
3.4.14.4
Propioxanthin A and B
-
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.14.4
0.053
-
Met-enkephalin
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.14.4
membrane
-
Rattus norvegicus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.14.4
Co2+
increase of activity
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.14.4
80000
86000
gel filtration
Rattus norvegicus
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.14.4
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.4.14.4
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.14.4
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.14.4
angiotensin II + H2O
-
36179
Rattus norvegicus
Asp-Arg + Val-Tyr + Ile-His + Pro-Phe
-
36179
Rattus norvegicus
?
3.4.14.4
Gly-Gly-Phe-Leu + H2O
-
36179
Rattus norvegicus
?
-
-
-
?
3.4.14.4
Met-enkephalin + H2O
-
36179
Rattus norvegicus
?
-
-
-
?
3.4.14.4
additional information
hydrolysis depending on type of amino acid and chain length
36179
Rattus norvegicus
?
-
-
-
?
3.4.14.4
Trp-Met-Asp-Phe-NH2 + H2O
-
36179
Rattus norvegicus
?
-
-
-
?
3.4.14.4
Tyr-Gly-Gly-Phe + H2O
-
36179
Rattus norvegicus
Tyr-Gly + Gly-Phe
-
36179
Rattus norvegicus
?
3.4.14.4
Tyr-Gly-Gly-Phe-Leu + H2O
-
36179
Rattus norvegicus
Tyr-Gly + Gly-Phe-Leu
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.4.14.4
monomer
1 * ?, SDS-PAGE
Rattus norvegicus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.4.14.4
4
40
-
Rattus norvegicus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.14.4
additional information
-
additional information
-
Rattus norvegicus
3.4.14.4
13.2
-
Tyr-Gly-Gly-Phe-Leu
-
Rattus norvegicus
3.4.14.4
50
-
Gly-Gly-Phe-Leu
-
Rattus norvegicus
3.4.14.4
167
-
Trp-Met-Asp-Phe-NH2
-
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.14.4
6
6.5
substrate Met-enkephalin
Rattus norvegicus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.4.14.4
6.5
8
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.14.4
1,10-phenanthroline
-
Rattus norvegicus
3.4.14.4
EDTA
-
Rattus norvegicus
3.4.14.4
p-hydroxymercuribenzoate
-
Rattus norvegicus
3.4.14.4
Propioxanthin A and B
-
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.14.4
0.053
-
Met-enkephalin
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.14.4
membrane
-
Rattus norvegicus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.14.4
Co2+
increase of activity
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.14.4
80000
86000
gel filtration
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.14.4
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.14.4
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.14.4
angiotensin II + H2O
-
36179
Rattus norvegicus
Asp-Arg + Val-Tyr + Ile-His + Pro-Phe
-
36179
Rattus norvegicus
?
3.4.14.4
Gly-Gly-Phe-Leu + H2O
-
36179
Rattus norvegicus
?
-
-
-
?
3.4.14.4
Met-enkephalin + H2O
-
36179
Rattus norvegicus
?
-
-
-
?
3.4.14.4
additional information
hydrolysis depending on type of amino acid and chain length
36179
Rattus norvegicus
?
-
-
-
?
3.4.14.4
Trp-Met-Asp-Phe-NH2 + H2O
-
36179
Rattus norvegicus
?
-
-
-
?
3.4.14.4
Tyr-Gly-Gly-Phe + H2O
-
36179
Rattus norvegicus
Tyr-Gly + Gly-Phe
-
36179
Rattus norvegicus
?
3.4.14.4
Tyr-Gly-Gly-Phe-Leu + H2O
-
36179
Rattus norvegicus
Tyr-Gly + Gly-Phe-Leu
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.14.4
monomer
1 * ?, SDS-PAGE
Rattus norvegicus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.4.14.4
4
40
-
Rattus norvegicus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.14.4
additional information
-
additional information
-
Rattus norvegicus
3.4.14.4
13.2
-
Tyr-Gly-Gly-Phe-Leu
-
Rattus norvegicus
3.4.14.4
50
-
Gly-Gly-Phe-Leu
-
Rattus norvegicus
3.4.14.4
167
-
Trp-Met-Asp-Phe-NH2
-
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.14.4
6
6.5
substrate Met-enkephalin
Rattus norvegicus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.4.14.4
6.5
8
-
Rattus norvegicus