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Literature summary extracted from

  • Ghosh, M.; Grunden, A.M.; Dunn, D.M.; Weiss, R.; Adams, M.W.W.
    Characterization of native and recombinant forms of an unusual cobalt-dependent proline dipeptidase (prolidase) from the hyperthermophilic archaeon Pyrococcus furiosus (1998), J. Bacteriol., 180, 4781-4789.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.13.9 expression in Escherichia coli Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.9 2.8
-
 Met-Pro native prolidase Pyrococcus furiosus
3.4.13.9 3
-
 Leu-Pro native prolidase Pyrococcus furiosus
3.4.13.9 3.3
-
 Met-Pro recombinant prolidase Pyrococcus furiosus
3.4.13.9 4.2
-
 Val-Pro native prolidase Pyrococcus furiosus
3.4.13.9 8.3
-
 Ala-Pro native prolidase Pyrococcus furiosus
3.4.13.9 20
-
 Phe-Pro native prolidase Pyrococcus furiosus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.13.9 Co2+ requires Co2+ in addition to one cobalt atom bound per subunit Pyrococcus furiosus
3.4.13.9 Mn2+ Co2+ can be replaced by Mn2+ Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.13.9 42000
-
 2 * 42000, SDS-PAGE Pyrococcus furiosus
3.4.13.9 100000
-
 gel filtration Pyrococcus furiosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.9 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.13.9 native and recombinant form Pyrococcus furiosus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.13.9 630
-
 native enzyme Pyrococcus furiosus
3.4.13.9 1360
-
 recombinant enzyme Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.9 L-Ala-Pro + H2O
-
 Pyrococcus furiosus L-Ala + Pro
-
 ?
3.4.13.9 L-Phe-Pro + H2O
-
 Pyrococcus furiosus L-Phe + Pro
-
 ?
3.4.13.9 L-Val-Pro + H2O
-
 Pyrococcus furiosus L-Val + Pro
-
 ?
3.4.13.9 Leu-Pro + H2O
-
 Pyrococcus furiosus Leu + Pro
-
 ?
3.4.13.9 Met-Pro + H2O
-
 Pyrococcus furiosus Met + Pro
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.13.9 dimer 2 * 42000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.13.9 100
-
 substrate Met-Pro Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.13.9 7
-
 substrate Met-Pro Pyrococcus furiosus