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Literature summary extracted from
- Purification and properties of membrane-bound aminopeptidase P from rat lung (1995), Biochemistry, 34, 11227-11236.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.11.9 | polyethylene glycol 20000 improves stability in the pH-range 4.5-7.0 | Rattus norvegicus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.9 | 1,10-phenanthroline | - |
Rattus norvegicus |  |
| 3.4.11.9 | 2-mercaptoethanol | - |
Rattus norvegicus | |
| 3.4.11.9 | 2-mercaptomethyl-3-guanidinoethylthiopropanoic acid | - |
Rattus norvegicus | |
| 3.4.11.9 | 4-chloromercuriphenyl sulfonic acid | - |
Rattus norvegicus | |
| 3.4.11.9 | apstatin | - |
Rattus norvegicus | |
| 3.4.11.9 | captopril | - |
Rattus norvegicus | |
| 3.4.11.9 | Co2+ | 1.5 mM CoCl2, complete inhibition | Rattus norvegicus | |
| 3.4.11.9 | dithiothreitol | - |
Rattus norvegicus | |
| 3.4.11.9 | EDTA | - |
Rattus norvegicus | |
| 3.4.11.9 | enalaprilat | inhibition only in presence of Mn2+ | Rattus norvegicus | |
| 3.4.11.9 | N-[1-(R,S)-carboxy-(2-phenylethyl)]-thiopropanoic acid | - |
Rattus norvegicus | |
| 3.4.11.9 | NaCl | 2 M, complete inhibition | Rattus norvegicus | |
| 3.4.11.9 | ramiprilat | inhibition only in presence of Mn2+ | Rattus norvegicus | |
| 3.4.11.9 | Zn2+ | 10 mM, complete inhibition | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.9 | 0.021 | - |
bradykinin | - |
Rattus norvegicus | |
| 3.4.11.9 | 0.03 | - |
L-Arg-L-Pro-L-Pro-Gly | - |
Rattus norvegicus | |
| 3.4.11.9 | 0.032 | - |
Arg-Pro-Pro-Gly-Phe-Ser | - |
Rattus norvegicus | |
| 3.4.11.9 | 0.039 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe | - |
Rattus norvegicus | |
| 3.4.11.9 | 0.048 | - |
Arg-Pro-Pro-Gly-Phe | - |
Rattus norvegicus | |
| 3.4.11.9 | 0.051 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro | - |
Rattus norvegicus | |
| 3.4.11.9 | 0.15 | - |
Arg-homoPro-Pro-Ala-NH2 | - |
Rattus norvegicus | |
| 3.4.11.9 | 0.32 | - |
Gly-Pro-Hyp | without Mn2+ | Rattus norvegicus | |
| 3.4.11.9 | 0.35 | - |
Arg-Pro-Pro | without Mn2+ | Rattus norvegicus | |
| 3.4.11.9 | 0.36 | - |
Arg-Pro-Pro | in presence of 4 mM Mn2+ | Rattus norvegicus | |
| 3.4.11.9 | 1 | - |
Arg-Pro-Pro | - |
Rattus norvegicus | |
| 3.4.11.9 | 2 | - |
Gly-Pro-Hyp | in presence of 4 mM Mn2+ | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.11.9 | microsome | membrane-bound | Rattus norvegicus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.9 | Mn2+ | 0.3-0.4 mM, 4fold stimulation with Gly-Pro-Hyp as substrate | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.9 | 90000 | - |
x * 90000, SDS-PAGE | Rattus norvegicus |
| 3.4.11.9 | 220000 | - |
value depends on assay conditions, gel filtration | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.9 | Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.11.9 | side-chain modification | the enzyme contains at least 17% N-linked carbohydrate | Rattus norvegicus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.9 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.11.9 | lung | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.9 | additional information | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.9 | allostatin 1 + H2O | - |
Rattus norvegicus | Ala + ? | cleavage of the Ala1-Pro2 bond | ? | |
| 3.4.11.9 | Arg-homoPro-Pro-Ala-NH2 + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.11.9 | Arg-Pro-Pro + H2O | - |
Rattus norvegicus | Arg + Pro-Pro | - |
? | |
| 3.4.11.9 | Arg-Pro-Pro-Gly-Phe + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.11.9 | Arg-Pro-Pro-Gly-Phe-Ser + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.11.9 | Arg-Pro-Pro-Gly-Phe-Ser-Pro + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.11.9 | Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O | - |
Rattus norvegicus | Arg + Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | - |
? | |
| 3.4.11.9 | Gly-Pro-Hyp + H2O | - |
Rattus norvegicus | Gly + Pro-Hyp | - |
? | |
| 3.4.11.9 | L-Arg-L-Pro-L-Pro-Gly + H2O | - |
Rattus norvegicus | L-Arg + L-Pro-L-Pro-Gly | - |
? | |
| 3.4.11.9 | additional information | no cleavage of human erythropoietin | Rattus norvegicus | ? | - |
? | |
| 3.4.11.9 | neuropeptide Y + H2O | - |
Rattus norvegicus | Tyr + ? | cleavage of the Try1-Pro2 bond | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.11.9 | ? | x * 90000, SDS-PAGE | Rattus norvegicus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.9 | 55 | - |
pH 6.8, 30 min, 0.1 M potassium phosphate buffer, stable up to | Rattus norvegicus |
| 3.4.11.9 | 70 | 75 | pH 6.8, 30 min, complete loss of activity | Rattus norvegicus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.9 | 11 | - |
Arg-Pro-Pro-Gly-Phe-Ser | - |
Rattus norvegicus | |
| 3.4.11.9 | 12 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg | - |
Rattus norvegicus | |
| 3.4.11.9 | 13 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe | - |
Rattus norvegicus | |
| 3.4.11.9 | 15 | - |
Arg-Pro-Pro-Gly-Phe | - |
Rattus norvegicus | |
| 3.4.11.9 | 16 | - |
Arg-Pro-Pro-Gly-Phe-Ser-Pro | - |
Rattus norvegicus | |
| 3.4.11.9 | 17 | - |
L-Arg-L-Pro-L-Pro-Gly | - |
Rattus norvegicus | |
| 3.4.11.9 | 48 | - |
Gly-Pro-Hyp | - |
Rattus norvegicus | |
| 3.4.11.9 | 69 | - |
Gly-Pro-Hyp | in presence of 4 mM Mn2+ | Rattus norvegicus | |
| 3.4.11.9 | 150 | - |
Arg-Pro-Pro | - |
Rattus norvegicus | |
| 3.4.11.9 | 180 | - |
Arg-Pro-Pro | in presence of 4 mM Mn2+ | Rattus norvegicus | |
| 3.4.11.9 | 230 | - |
Arg-Pro-Pro | without 4 mM Mn2+ | Rattus norvegicus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.9 | 6.8 | 7.5 | hydrolysis of Arg-Pro-Pro | Rattus norvegicus |
| 3.4.11.9 | 7 | - |
hydrolysis of bradykinin(1-8), 0.5 mM, in both 0.1 M Hepes buffer or 0.1 M potassium phosphate buffer | Rattus norvegicus |
| 3.4.11.9 | 8 | - |
6 mM imidazole buffer, Arg-Pro-Pro as substrate | Rattus norvegicus |
| 3.4.11.9 | 9 | - |
11 mM imidazole buffer, hydrolysis of Arg-Pro-Pro | Rattus norvegicus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.9 | 4.5 | 7 | polyethylene glycol 20000 improves stability in the pH-range 4.5-7.0 | Rattus norvegicus |
| 3.4.11.9 | 7 | 10.5 | optimal stability | Rattus norvegicus |
| 3.4.11.9 | 8.5 | 10 | at 37°C, 2 h, most stable at | Rattus norvegicus |
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