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Literature summary extracted from

  • Hua, G.; Tsukamoto, K.; Taguchi, R.; Tomita, M.; Miyajima, S.; Ikezawa, H.
    Characterization of aminopeptidase N from the brush border membrane of the larvae midgut of silkworm, Bombyx mori as a zinc enzyme [published erratum appears in Biochim Biophys Acta 1998 Sep 8;1387(1-2):486 (1998), Biochim. Biophys. Acta, 1383, 301-310.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 1,10-phenanthroline 97% inhibition after 10 min at 10 mM Bombyx mori
3.4.11.2 actinonin IC50: 148 microM Bombyx mori
3.4.11.2 amastatin IC50: 7.6 microM Bombyx mori
3.4.11.2 bestatin poor inhibitor, IC50: 3.25 mM Bombyx mori
3.4.11.2 Cu2+
-
 Bombyx mori
3.4.11.2 EDTA only 27% inhibition at 300 mM, 50% inhibition after incubation for 12 h with 300 mM Bombyx mori
3.4.11.2 probestin IC50: 74 microM Bombyx mori
3.4.11.2 Zn2+ inhibits at concentrations above 0.1 mM Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.2 0.222
-
 Leu-4-nitroanilide
-
 Bombyx mori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.2 membrane brush border membrane Bombyx mori 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 Ca2+ increase of activity Bombyx mori
3.4.11.2 Mg2+ increase of activity Bombyx mori
3.4.11.2 Zn2+ increase of activity at concentrations below 0.1 mM Bombyx mori
3.4.11.2 Zn2+ content: 0.72 mol/mol protein Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.2 aminoacyl peptide Bombyx mori
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Bombyx mori
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 midgut larvae Bombyx mori
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 aminoacyl peptide
-
 Bombyx mori ?
-
 ?
3.4.11.2 Leu-p-nitroanilide + H2O
-
 Bombyx mori Leu + p-nitroaniline
-
 ?

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.11.2 0.0076
-
 IC50: 7.6 microM Bombyx mori amastatin
3.4.11.2 0.074
-
 IC50: 74 microM Bombyx mori probestin
3.4.11.2 0.148
-
 IC50: 148 microM Bombyx mori actinonin
3.4.11.2 3.25
-
 poor inhibitor, IC50: 3.25 mM Bombyx mori bestatin