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Literature summary extracted from
- Glutathione-degrading enzymes of microvillus membranes (1982), J. Biol. Chem., 257, 6322-6327.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.2 | dithiothreitol | no significant inhibition by thiol compounds | Rattus norvegicus |  |
| 3.4.13.19 | 1,10-phenanthroline | ZnSO4 restores activity | Rattus norvegicus | |
| 3.4.13.19 | DTT | - |
Rattus norvegicus | |
| 3.4.13.19 | thiol compounds | - |
Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.2 | 1 | - |
L-alpha-aminobutyryl-Gly | - |
Rattus norvegicus | |
| 3.4.11.2 | 2.5 | - |
Cys-Gly | - |
Rattus norvegicus | |
| 3.4.11.2 | 3.4 | - |
Ala-Gly | - |
Rattus norvegicus | |
| 3.4.11.2 | 4.8 | - |
L-cystinyl-bis-Gly | - |
Rattus norvegicus | |
| 3.4.13.19 | 0.6 | - |
L-cystinyl-bis-Gly | - |
Rattus norvegicus | |
| 3.4.13.19 | 0.8 | - |
L-Ala-Gly | - |
Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.11.2 | membrane | - |
Rattus norvegicus | 16020 | - |
| 3.4.13.19 | microvillus | - |
Rattus norvegicus | 5902 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.13.19 | Zinc | enzyme contains about 2 Zn per subunit | Rattus norvegicus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.2 | 130000 | - |
SDS-PAGE | Rattus norvegicus |
| 3.4.13.19 | 50000 | - |
2 * 50000, SDS-PAGE | Rattus norvegicus |
| 3.4.13.19 | 105000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.2 | aminoacyl peptide | Rattus norvegicus | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.2 | Rattus norvegicus | - |
- |
- |
| 3.4.13.19 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.2 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.11.2 | epididymis | - |
Rattus norvegicus | - |
| 3.4.11.2 | jejunum | - |
Rattus norvegicus | - |
| 3.4.11.2 | kidney | - |
Rattus norvegicus | - |
| 3.4.13.19 | kidney | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.2 | 0.6 | - |
L-cystinyl-bis-Gly as substrate | Rattus norvegicus |
| 3.4.11.2 | 6.9 | - |
Cys-Gly as substrate | Rattus norvegicus |
| 3.4.11.2 | 9.1 | - |
L-alpha-aminobutyryl-Gly as substrate | Rattus norvegicus |
| 3.4.11.2 | 11.5 | - |
Leu-p-nitroanilide as substrate | Rattus norvegicus |
| 3.4.11.2 | 33.5 | - |
Ala-Gly as substrate | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.2 | Ala-Gly + H2O | - |
Rattus norvegicus | Ala + Gly | - |
? | |
| 3.4.11.2 | aminoacyl peptide | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.11.2 | Cys-Gly + H2O | - |
Rattus norvegicus | Cys + Gly | - |
? | |
| 3.4.11.2 | dipeptides + H2O | not: D-Ala-Gly, Val-Gly, Asp-Gly, Gly-D-Ala, Pro-containing peptides | Rattus norvegicus | amino acids | - |
? | |
| 3.4.11.2 | L-alpha-aminobutyryl-Gly + H2O | - |
Rattus norvegicus | L-alpha-aminobutyrate + Gly | - |
? | |
| 3.4.11.2 | L-cystinyl-bis-Gly + H2O | - |
Rattus norvegicus | 2 Gly + L-cysteine | - |
? | |
| 3.4.13.19 | Ala-Ala + H2O | - |
Rattus norvegicus | Ala + Ala | - |
? | |
| 3.4.13.19 | Ala-Met + H2O | - |
Rattus norvegicus | Ala + Met | - |
? | |
| 3.4.13.19 | Gly-Ala + H2O | L-Ala and D-Ala | Rattus norvegicus | Gly + Ala | - |
? | |
| 3.4.13.19 | Gly-Gly + H2O | - |
Rattus norvegicus | Gly + Gly | - |
? | |
| 3.4.13.19 | L-Ala-Gly + H2O | - |
Rattus norvegicus | L-Ala + Gly | - |
? | |
| 3.4.13.19 | L-cystinyl-bis-Gly + H2O | - |
Rattus norvegicus | L-Cys + Gly-Gly | - |
? | |
| 3.4.13.19 | L-cystinyl-bis-glycine + H2O | - |
Rattus norvegicus | ? | - |
? | |
| 3.4.13.19 | Lys-Gly + H2O | - |
Rattus norvegicus | Lys + Gly | - |
? | |
| 3.4.13.19 | Met-Met + H2O | - |
Rattus norvegicus | Met + Met | - |
? | |
| 3.4.13.19 | additional information | no hydrolysis of L-Asp-Gly | Rattus norvegicus | ? | - |
? | |
| 3.4.13.19 | additional information | no hydrolysis of proline-containing peptides | Rattus norvegicus | ? | - |
? | |
| 3.4.13.19 | additional information | no hydrolysis of glutathione | Rattus norvegicus | ? | - |
? | |
| 3.4.13.19 | additional information | no hydrolysis of L-Val-Gly | Rattus norvegicus | ? | - |
? | |
| 3.4.13.19 | additional information | no hydrolysis of D-Ala-Gly | Rattus norvegicus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.13.19 | dimer | 2 * 50000, SDS-PAGE | Rattus norvegicus |
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