BRENDA - Enzyme Database

Purification and characterization of aminopeptidase M from muscle and mucosa of the pig intestine

Terashima, H.; Bunnett, N.W.; J. Gastroenterol. 30, 696-704 (1995) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.11.2
1,10-phenanthroline
-
Sus scrofa
3.4.11.2
actinonin
almost complete inhibition at 0.1 mM
Sus scrofa
3.4.11.2
amastatin
almost complete inhibition at 0.001 mM
Sus scrofa
3.4.11.2
bestatin
almost complete inhibition at 0.1 mM
Sus scrofa
3.4.11.2
EDTA
27-30% inhibition at 1 mM
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.11.2
membrane
mucosal membrane
Sus scrofa
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.4.11.2
aminoacyl peptide
Sus scrofa
-
?
-
-
?
3.4.11.2
enkephalin
Sus scrofa
may be involved in enkephalin neurotransmitter inactivation
?
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.11.2
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.4.11.2
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.11.2
intestine
-
Sus scrofa
-
3.4.11.2
muscle
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.11.2
3
3.9
Leu5-enkephalin as substrate
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.11.2
aminoacyl peptide
-
35867
Sus scrofa
?
-
-
-
?
3.4.11.2
enkephalin
may be involved in enkephalin neurotransmitter inactivation
35867
Sus scrofa
?
-
-
-
?
3.4.11.2
Leu5-enkephalin + H2O
-
35867
Sus scrofa
tyrosin + (2-5)Leu5-enkephalin
-
35867
Sus scrofa
?
3.4.11.2
Met5-enkephalin + H2O
-
35867
Sus scrofa
tyrosin + (2-5)Met5-enkephalin
-
35867
Sus scrofa
?
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.11.2
1,10-phenanthroline
-
Sus scrofa
3.4.11.2
actinonin
almost complete inhibition at 0.1 mM
Sus scrofa
3.4.11.2
amastatin
almost complete inhibition at 0.001 mM
Sus scrofa
3.4.11.2
bestatin
almost complete inhibition at 0.1 mM
Sus scrofa
3.4.11.2
EDTA
27-30% inhibition at 1 mM
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.11.2
membrane
mucosal membrane
Sus scrofa
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
3.4.11.2
aminoacyl peptide
Sus scrofa
-
?
-
-
?
3.4.11.2
enkephalin
Sus scrofa
may be involved in enkephalin neurotransmitter inactivation
?
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.11.2
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.11.2
intestine
-
Sus scrofa
-
3.4.11.2
muscle
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.11.2
3
3.9
Leu5-enkephalin as substrate
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.11.2
aminoacyl peptide
-
35867
Sus scrofa
?
-
-
-
?
3.4.11.2
enkephalin
may be involved in enkephalin neurotransmitter inactivation
35867
Sus scrofa
?
-
-
-
?
3.4.11.2
Leu5-enkephalin + H2O
-
35867
Sus scrofa
tyrosin + (2-5)Leu5-enkephalin
-
35867
Sus scrofa
?
3.4.11.2
Met5-enkephalin + H2O
-
35867
Sus scrofa
tyrosin + (2-5)Met5-enkephalin
-
35867
Sus scrofa
?