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Literature summary extracted from
- Leucine aminopeptidase and other N-terminal exopeptidases (1971), The Enzymes, 2nd Ed. (Boyer, P. D. , Lardy, H. , Myrbäck, K. , eds. ), 3, 81-118.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.1 | 1-butanol | - |
Bos taurus |  |
| 3.4.11.1 | 1-butanol | - |
Sus scrofa | |
| 3.4.11.1 | aliphatic alcohols | methanol to n-hexanol | Bos taurus | |
| 3.4.11.1 | aliphatic alcohols | methanol to n-hexanol | Sus scrofa | |
| 3.4.11.1 | alpha-keto-isocaproamide | - |
Bos taurus | |
| 3.4.11.1 | alpha-keto-isocaproamide | - |
Sus scrofa | |
| 3.4.11.1 | Cu2+ | - |
Bos taurus | |
| 3.4.11.1 | Cu2+ | - |
Sus scrofa | |
| 3.4.11.1 | D,L-Alpha-hydroxyisocaproamide | - |
Bos taurus | |
| 3.4.11.1 | D,L-Alpha-hydroxyisocaproamide | - |
Sus scrofa | |
| 3.4.11.1 | glycerol | - |
Bos taurus | |
| 3.4.11.1 | glycerol | - |
Sus scrofa | |
| 3.4.11.1 | Hg2+ | - |
Bos taurus | |
| 3.4.11.1 | Hg2+ | - |
Sus scrofa | |
| 3.4.11.1 | Isocaproic acid | - |
Bos taurus | |
| 3.4.11.1 | Isocaproic acid | - |
Sus scrofa | |
| 3.4.11.1 | L-leucine | competitive | Bos taurus | |
| 3.4.11.1 | L-leucine | competitive | Sus scrofa | |
| 3.4.11.1 | L-Leucinol | - |
Bos taurus | |
| 3.4.11.1 | L-Leucinol | - |
Sus scrofa | |
| 3.4.11.1 | Pb2+ | - |
Bos taurus | |
| 3.4.11.1 | Pb2+ | - |
Sus scrofa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.1 | 0.51 | - |
L-Leu-L-Val | - |
Sus scrofa | |
| 3.4.11.1 | 1.56 | - |
L-Leu-benzyl ester | - |
Sus scrofa | |
| 3.4.11.1 | 4.02 | - |
leucinamide | similar value | Sus scrofa | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.11.2 | microsome | - |
Sus scrofa | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.1 | Mg2+ | activation | Sus scrofa | |
| 3.4.11.1 | Mg2+ | activation | Bos taurus | |
| 3.4.11.1 | Mn2+ | activation | Sus scrofa | |
| 3.4.11.1 | Mn2+ | activation | Bos taurus | |
| 3.4.11.1 | Zn2+ | - |
Sus scrofa | |
| 3.4.11.1 | Zn2+ | - |
Bos taurus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.2 | aminoacyl peptide | Sus scrofa | - |
? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.1 | Bos taurus | - |
- |
- |
| 3.4.11.1 | Sus scrofa | - |
hog | - |
| 3.4.11.2 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.11.1 | overview procedures | Sus scrofa |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.4.11.1 | release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low | mechanism | Sus scrofa | |
| 3.4.11.1 | release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low | mechanism | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.11.1 | lens | - |
Bos taurus | - |
| 3.4.11.1 | pancreas | - |
Sus scrofa | - |
| 3.4.11.2 | kidney | - |
Sus scrofa | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.4.11.1 | frozen, pH 7-9, presence of MgCl2, indefinitely | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.1 | L-Leu-benzyl ester + H2O | - |
Sus scrofa | L-Leu + benzylalcohol | - |
? | |
| 3.4.11.1 | L-leucine amide + H2O | - |
Sus scrofa | L-leucine + NH3 | - |
? | |
| 3.4.11.1 | Leu-Val + H2O | - |
Sus scrofa | Leu + Val | - |
? | |
| 3.4.11.1 | additional information | use in sequence determination | Sus scrofa | ? | - |
? | |
| 3.4.11.1 | additional information | use in sequence determination | Bos taurus | ? | - |
? | |
| 3.4.11.1 | additional information | no hydrolysis of Xaa-Pro bond | Sus scrofa | ? | - |
? | |
| 3.4.11.1 | additional information | no hydrolysis of Xaa-Pro bond | Bos taurus | ? | - |
? | |
| 3.4.11.2 | aminoacyl peptide | - |
Sus scrofa | ? | - |
? | |
| 3.4.11.2 | aminoacyl peptide + H2O | fast hydrolysis with Ala in N-terminal position | Sus scrofa | alanyl peptides + H2O | - |
? | |
| 3.4.11.2 | aminoacyl peptide + H2O | free alpha-amino or alpha-imino group required | Sus scrofa | alanyl peptides + H2O | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.1 | 9 | 9.4 | - |
Sus scrofa |
| 3.4.11.1 | 9 | 9.4 | - |
Bos taurus |
| 3.4.11.2 | 7 | 7.5 | - |
Sus scrofa |
| 3.4.11.2 | 9 | - |
at high substrate concentrations | Sus scrofa |
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